kappa-Carrageenan is a polysaccharide from red seaweed which gets precipitated by K+ ions and dissolves again in water. This smart, K(+)-responsive polymer was found to selectively bind pullulanase activity from Bacillus acidopullulyticus. Gel filtration on Sephadex G-200 showed the formation of the polymer-pullulanase complex at the pre-precipitation stage. On the other hand, phospholipase D, ...

Two processes permit the urine pH and the medullary interstitial pH to remain in an "ideal range" to minimize the risk of forming kidney stones. First, a medullary shunt for NH(3) maintains the urine pH near 6.0 to minimize uric acid precipitation when distal H(+) secretion is high. Second, excreting dietary alkali excreting alkali as a family of organic anions--including citrate--rather than a...

The staining of thin sections of Osmium tetroxide (OsO4)-fixed biological materials with the lead hydroxide preparat ion of Watson (5) or with semisaturated solutions of lead subacetate (1) represents a considerable advance in electron microscope technique. The contrast of the image viewed in the electron microscope is so far enhanced that accurate focusing is now possible at appreciably higher...

Changes in the concentration of interstitial iron in multicrystalline silicon wafers after high temperature annealing (900oC) have been monitored by carrier lifetime measurements. Two cooling rates were investigated. The first was considered ‘fast’, meaning the interstitial Fe had no time to diffuse to precipitation sites, and should therefore be frozen-in, despite being far above the solubilit...

5. Inorganic Polyphosphate Chemistry 4699 5.1. History 4699 5.2. Definition 4699 5.3. Formation 4699 5.4. Hydrolytic Degradation (Hydrolysis) 4699 5.5. Analytical Methods 4700 5.5.1. Chromatography 4700 5.5.2. Hydrolytic Degradation Assay 4700 5.5.3. Titration 4700 5.5.4. Colorimetry 4700 5.5.5. Solubility Fractionation 4700 5.5.6. Gel Electrophoresis 4700 5.5.7. P NMR 4700 5.5.8. Infrared 4700...

HutB, the periplasmic hemin binding protein of Photobacterium damselae subsp. piscicida, was produced as a recombinant protein. UV-Vis spectrophotometrical analysis showed absorption spectral changes in hemin upon mixing it with the recombinant protein, indicating complex formation. Spectrophotometric titration of HutB with hemin showed saturation at a heme/HutB ratio of 1:1 and a binding affin...

The evaluation of texture as a function of recrystallization has been characterized for directly cold rolled Al-6Mg, Al-6Mg-0.4Sc and Al-6Mg-0.4Sc-0.2Zr alloys. Samples were annealed isothermally at 400 °C for 1 to 240 minutes to allow recrystallization. Recrystallization kinetics of the alloys is analyzed from the micro-hardness variation. Isothermally annealed samples of aluminum alloys were ...

MEASUREMENT of the titration constants of simple peptides is of biochemical interest for two reasons. Firstly, it provides information necessary for careful titrimetric estimation of amino-N in biological fluids or enzymic proteolysates [Richardson, 1934, 1, 2]. Secondly, it facilitates interpretation of protein titration curves by helping to define the change of titration constant which result...