Male patients with NPC1 heterozygous mutation (Npc1+/-) are prone to obesity and diabetes, yet the mechanism remains unclear. In this study, male Npc1+/- mice (C57BL/6C-Npc1) were used evaluate effects of combined 60% high-fat diet (HFD) on glucolipid metabolism, cholesterol accumulation, islet dysfunction β-cell dedifferentiation. Compared HFD-Npc1+/+ or fed low-fat (LFD), body weight HFD-Npc1...

We recently reported that the direct antitumor effectors in the liver induced by alpha-galactosylceramide (alpha-GalCer) are NK cells that are activated by the IFN-gamma produced from NK1.1 Ag(+) T cells (NKT cells) specifically stimulated with alpha-GalCer, whereas NKT cells cause hepatocyte injury through the Fas-Fas ligand pathway. In the present study, we investigated how mouse age affects ...

Cell therapy may be a novel and effective treatment strategy for liver diseases, replacing liver transplantation. The potential of two alternative cell types (hepatic progenitor/stem cells and mature hepatocytes) has not yet been fully assessed; the issues of low amplification efficiency and recovery function remain to be resolved. In this study, we investigated the proliferation, differentiati...

Hepatic stellate cells (HSCs) play a key role in liver fibrosis. Succinate and succinate receptor (GPR91) signaling pathway are involved the activation, proliferation, migration of HSCs. We investigated whether may induce hepatic The mice were randomly divided into 2 groups —the control group (chow diet-fed mice, n = 26) sodium (2% + chow diet, 38). Each diet was provided for 16 weeks. Mice adm...

Abstract Background: Cancers do not exist in isolation, surrounding tumours are supportive cells, which create the microenvironment cancer cells reside. In prostate (PCa), androgen receptor (AR) signalling fibroblasts is strikingly distinct from that within PCa and has specific functions to produce, maintain, modulate extracellular matrix (ECM) surrounds interacts with cells. The of metastatic ...

Abstract Background Protopine is an isoquinoline alkaloid that possesses various biological activities including the anti-tumour activity. However, effects of protopine on liver carcinoma cells are still elusive. The aim this study to examine both in vitro and vivo. Methods MTT assay was performed measure cell viability. Wound healing transwell assays were conducted assess motility cells. Cellu...

Repressor and activator protein (Rap1) directly regulates nuclear factor-κB (NF-κB) dependent signaling, which contributes to hepatic IRI. We here intended to investigate the effect of Rap1 in hepatic ischemia reperfusion injury (IRI) and to explore the underlying mechanisms. The association of Rap1 expression with hepatic inflammatory response were investigated in both human and rat liver tran...

AMP-activated protein kinase (AMPK) plays a key role in integrating metabolic pathways in response to energy demand. We identified a mutation in the γ1 subunit (γ1D316A) that leads to activation of AMPK. We generated mice with this mutation to study the effect of chronic liver-specific activation of AMPK in vivo. Primary hepatocytes isolated from these mice have reduced gluconeogenesis and fatt...

Secondary leukemia occurs long after remission of the primary tumor with a history of chemo and radiation therapy; however, the mechanism of secondary leukemogenesis is not fully understood. In this study, we demonstrate cases of secondary T-cell leukemia in mice after complete regression of xenograft primary glioblastoma independent of chemo and radiotherapy. Recently, antiangiogenic therapy, ...