The application of long-chained peptides (+30 aa) and relatively short proteins (<300 has experienced an increasing interest in recent years. However, a reliable production platform is still missing since manufacturing challenged by inherent problems such as mis-folding, aggregation, low yields. And neither chemical synthesis nor available recombinant approaches are effective efficient. ...

Abstract In Germany alone, more than 5·10 6 tons of municipal green waste is produced each year. So far, this material not used in an economically worthwhile way. work, grass clippings and tree pruning as examples were utilized feedstock for the microbial production platform chemicals. A pretreatment procedure depending on moisture lignin content biomass was developed. The suitability press jui...

Heterologous expression has become a powerful tool for studying microbial biosynthetic gene clusters (BGCs). Here, we extend the transformation-associated recombination cloning and heterologous expression platform for microbial BGCs to include Gram-negative proteobacterial expression hosts. Using a broad-host-range expression platform, we test the implicit assumption that biosynthetic pathways ...