We isolated the 5’ upstream promoter regions of the fruit-type sucrose synthase (SS) gene from tomato and Japanese pear by inverse PCR. The 5’ region of the tomato SS gene (TOMSSF) contained an intron approximately 1.6 kbp long in the 5’untranslated region, whereas the equivalent 5’ region in the Japanese pear SS gene (PypSUS1) had no intron. Each region was fused to the β-glucuronidase (GUS) g...

Professional skills expected from science, technology, engineering and math (STEM) students are well-aligned with the broad learning outcomes defined by the Accreditation Board of Engineering and Technology (ABET). While acquisition of professional skills in our graduates is increasingly crucial due to global competition and intensifying pressures on companies, the absence of a robust assessmen...

When multiple linear regression is used to develop a prediction model, sample size must be large enough to ensure stable coefficients. If the derivation sample size is inadequate, the model may not predict well for future subjects. The precision efficacy analysis for regression (PEAR) method uses a crossvalidity approach to select sample sizes such that models will predict as well as possible i...

It is well-known that for most spherical rubber balloons the pressure versus volume curve associated with uniform inflation has an N-shape (the pressure increases rapidly to a maximum, falls to a minimum, and subsequently increases monotonically), and that somewhere along the descending branch of this curve the spherical shape may bifurcate into a pear shape through localized thinning near one ...

A cultivation-independent approach based on polymerase chain reaction (PCR)-amplified partial small subunit rRNA genes was used to characterize bacterial populations in the surface soil of a commercial pear orchard consisting of different pear cultivars during two consecutive growing seasons. Pyrus communis L. cvs Blanquilla, Conference, and Williams are among the most widely cultivated cultiva...

Thirteen isolates of Botryodiplodia theobromae collected from pear varieties grown in various regions of Punjab were studied for morphological, pathological and molecular characterization. The mycelial growth of B. theobromae isolates was classified as fluffy or depressed, uniform to irregular and cottony white turning to black. Colony growth rate varied from 19.1 to 24.9 mm per day. Pycnidia w...

The aim of this study was to quantify mucilages, pectins, hemicelluloses, and cellulose of nopalitos (edible, as vegetable, young cladodes of flat-stemmed spiny cacti) of most consumed Mexican cultivars, and sweet and acid cactus pear fruits of Opuntia spp. The hypothesis is that, regardless of their unavailable polysaccharides diversity, nopalitos and cactus pear fruits are rich sources of sol...

Biochemistry 218 Antiretroviral drug therapy has been one of the few promising areas in the fight against HIV, contributing to a significant reduction in mortality and morbidity due to HIV in the developed world. However, successful use of antiretroviral drugs is plagued by the phenomenon of drug resistance. A crucial challenge facing antiretroviral therapy is the development of accurate, conve...

The aim of the study is to identify frequency occurrence various allelic variants and genotypes beta-casein in cows Kholmogory breed their relationship with dairy productivity. tasks research are genotyping cattle by locus establishing its connection qualitative quantitative indicators As objects for there were taken 150 1st, 2nd 3rd lactation. An allele-specific variant PCR method (AS-PCR) was...