mdash; Plant regeneration from immature intergeneric embryos through direct callus induction was investigated in the case of hybridization between T aestivum cv Chinese Spring (2n = 6x = 42, AABBDD) and A cristatum 4x with one B chromosome (2n = 4x = 28 + 1 B, PPPP). Twenty-three abnormal immature hybrid embryos were directly cultured on MS solid medium with 2 mg / L 2,4-D to induce calli which...

Three trials have been set up in Côte d’Ivoire to analyse the growth of six-year-old microcuttings produced from seedlings, 45-month-old somatic embryos of PB 260 and PR 107 and 31-month-old microcuttings of IRCA 18. The results show that Hevea in vitro plantlets produced by somatic embryogenesis or microcutting consistently grow as well as if not better than conventional budded plants. The dif...

Plectranthus amboinicus (Lour.) Spreng is a perennial plant from the mint family with aromatic, succulent leaves and several health benefits. Multiple shoot regeneration was accomplished in vitro using nodal segments (NS) explants of P. pretreated 0, 0.5, 5, 25, 50, 100 μM thidiazuron (TDZ) for 4 h, then transferred to growth regulator-free media. After 8 weeks growth, NS pre-treated 25 TDZ h T...

An effective protocol for the plant regeneration via direct and indirect organogenesis has been developed from leaf explants of Asystasia gangetica (L.), cultured on Murashige Skoog (MS) medium supplemented with various concentrations combinations auxin cytokinins. Approximately 86% produced shoots MS containing 0.5 mg L−1 6-benzyladenine (BA) 10 μg Triacontanol (TRIA) a maximum 4.82 ± 0.29 per...

Abstract The paper presents a technique for micropropagation of endangered in Europe and extinct Poland Pulsatilla vulgaris ex situ conservation the genetic resources. Genotype-dependent induction somatic embryogenesis rooting was revealed series two experiments (I II) performed under same experimental conditions. Shoot tips seedlings were best explants both Murashige Skoog (MS) medium suppleme...

In vitro callogenesis was achieved from leaf explants of Boerhaavia diffusa Linn. on basal medium supplemented with different concentrations of BAP, NAA, Kn and IAA. Regeneration of shoot buds were observed on MS medium supplemented with BAP and NAA at different concentrations. In some of the replicates the shoot buds were elongated and terminated in flower buds. In vitro flowering occurred wit...

Plants were regenerated from encapsulated shoot tips of pointed gourd. Shoot tips isolated from multiple shoot cultures of AM-8 and AM-15 cultivars of pointed gourd were encapsulated in sodium alginate beads. For germination and shoot proliferation, encapsulated shoot tips (artificial seed) were cultured in MS basal medium containing different concentrations and combinations of BAP and NAA. Use...

Genome editing is a powerful technique for genome modification in molecular research and crop breeding, and has the great advantage of imparting novel desired traits to genetic resources. However, the genome editing of fruit tree plantlets remains to be established. In this study, we describe induction of a targeted gene mutation in the endogenous apple phytoene desaturase (PDS) gene using the ...

The transformation of coffee plantlets with the cry1ac gene of Bacillus thuringiensis was achieved by biolistic using either the whole pUBC plasmid or only the ubi-cry1ac-nos genetic cassette. The cry1ac gene was inserted into coffee plants in order to confer resistance to the leaf miner Leucoptera coffeella, an insect responsible for considerable losses in coffee crops. Bearing in mind that th...