Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by the accumulation and aggregation of extracellular amyloid β (Aβ) peptides and intracellular aggregation of hyper-phosphorylated tau protein. Recent evidence indicates that accumulation and aggregation of intracellular amyloid β peptides may also play a role in disease pathogenesis. This would suggest that intra...

Neurodegenerative diseases are characterized by intra- and/or extracellular protein aggregation and oxidative stress. Intense attention has been paid to whether protein aggregation itself contributes to abnormal production of free radicals and ensuing cellular oxidative damage. Although this question has been investigated in the context of extracellular protein aggregation, it remains unclear w...

a binding study of nickel ions by a new recombinant human growth hormone (hgh), produced as an injected drug, has been done at 27˚c in nacl solution (50 mm) using an isothermal titration calorimetry. there is a set of three identical and non-interacting binding sites for nickel ions. the intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 40 μm and -16.5 kj/mol, re...

abstractintroduction: cancer is caused by genetic abnormalities, such as mutation of ontogenesis or tumor suppressor genes which alter downstream signaling pathways and protein-protein interactions. comparison of protein interactions in cancerous and normal cells can be of help in mechanisms of disease diagnoses and treatments. methods: we constructed protein interaction networks of cancerous a...

This study presents the design and characterisation of casein−whey protein suspensions (8.0/10.0% (w/w) casein 2.0/2.5% whey protein) mixed with dairy fat (1.0, 2.5 5.0% total fat) processed via pH−temperature-route in preparation for 3D-printing. Mechanical treatment was applied to significantly decrease particle size milk globules increase surface area, creating small (<1 μm) covered proteins...

Protein aggregation is a common characteristic of several human diseases such as Alzheimer’s disease. Recent evidence has indicated that the peptides p53 also marked in cancer cells. The aim this study was to correlate Thioflavin T (ThT) data with different cellular viability assays (Neutral Red and MTT) SH-SY5Y neuroblastoma cells HT-29 colon treated doxorubicin, classical antineoplastic agent...

Protein aggregation and amyloid fibril deposits are characteristic features of more than twenty pathologic conditions characterized by plaque deposition in the central nervous system. Recent studies point out relationships between protein misfolding and numerous serious diseases. Despite different origins (sporadic, familial or transmissible), they are sometimes called conformational diseases t...

Prion diseases, like Alzheimer's disease and Parkinson disease, are rapidly progressive neurodegenerative disorders caused by misfolding followed by aggregation and accumulation of protein deposits in neuronal cells. Here we measure intramolecular polypeptide backbone reconfiguration as a way to understand the molecular basis of prion aggregation. Our hypothesis is that when reconfiguration is ...

S of yeast and mammalian prions introduced the idea that the protein aggregates can exist in multiple stable conformations that can be propagated by seeding. These conformational states (aka strains) were shown to have distinct physical (secondary structure, stability) and biological (cytotoxicity, infectivity) properties. For mammalian prions they were also tied to differences in disease patho...