the aim of this study was to produce a recombinant protein consisting of the catalytic and translocation domains of diphtheria toxin for its later application as a vaccine candidate against corynebacterium diphtheria. to achieve this goal, at first, the amino acid sequence of dt386 was used for prediction of t and b cell epitopes using on-line servers. the dt386 coding sequence was synthesized ...

background: nontypeable haemophilus influenzae (nthi) is a common cause of respiratory tract disease and initiates infection by colonization in nasopharynx. the haemophilus influenzae (h. influenzae) hap adhesin is an auto transporter protein that promotes initial interaction with human epithelial cells. hap protein contains a 110 kda internal passenger domain called “haps” and a 45 kda c-termi...

Background: The B-cell defect in X-linked agammaglobulinemia (XLA) is caused by mutations in the gene for Bruton's tyrosine kinase (BTK). BTK mutations result in deficient expression of BTK protein in peripheral blood monocytes. Methods: Using the anti-BTK monoclonal antibody (48-2H), a flow cytometric analysis of intra cytoplasmic BTK protein expression in monocytes was performed to identify I...

synchronization of rumen available protein and energy is one of the conceptual methods to increase the efficiency of utilization of nutrients by the ruminants the concept of synchronization energy and protein was first by jhonson, implying that maximum microbial protein synthesis could be achieved by matching the rate of organic matter and protein degradation. synchronization of rumen available...

Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...

Phytochemical constituents, such as total phenol, allicin and pyrovic acid as well as antioxidant activity of thirteen ecotypes of Allium hirtifolium Boiss. from different regions of Iran were evaluated. Average contents of total phenol, allicin and pyrovic acid of bulbs extracts per g fresh weight were 0.647 mg gallic acid equivalent (GAE), 2.127 mg and 84.412 µmol, respectively and antioxidan...

Objective(s) Staphylococcus aureus is a foremost source of numerous nosocomial and community acquired infections. Antibiotic therapy for vancomycin resistant S. aureus (VRSA) can not promise the eradication of infections. Since adhesion is the major route of infections, adhesin based vaccine could suppress S. aureus infections. Fibronectin binding protein A (FnBPA) and clumping factor A (ClfA)...