The denaturation of ribonuclease A by guanidine hydrochloride, lithium bromide, and lithium chloride and by mixed denaturants consisting of guanidine hydrochloride and one of the denaturants lithium chloride, lithium bromide, and sodium bromide was followed by difference spectral measurements at pH 4.8 and 25 degrees C. Both components of mixed denaturant systems enhance each other's effect in ...

It was shown that at low concentrations guanidine hydrochloride (GdnHCl) can cause aggregation of proteins in partially folded state and that fluorescent dye 1-anilinonaphthalene-8-sulfonic acid (ANS) binds with these aggregates rather than with hydrophobic clusters on the surface of protein in molten globule state. That is why the increase in ANS fluorescence intensity is often recorded in the...

Unlike ferrihemoglobin (Hb+), denatured carbonylhemoglobin (COHb) has almost the same absorption spectrum in the visible and near ultraviolet region as the native form; the very slight difference’appears only in the Soret band. Kinetic and equilibrium experiments under anaerobic conditions show that COHb is considerably more resistant to acid denaturation at 25’ than is Hb+ although the rates o...

Heats of thermal denaturation of chromatin core particles and core particles with covalently crosslinked histones were measured by differential scanning calorimetry. The additional stabilization of the nucleoprotein complex by crosslinking is not reflected in the transition enthalpy. The contribution of protein denaturation to the total heat was estimated by comparison of core particles with co...

introduction: at least 60% of the estimated 300,000 metric tons of tuna that are processed in iran areby-products which arebeingwasted and converted to non-human products as fish meal or fertilizers. therefore, a major challenge facing the tuna canning industry is to find the new processes to utilize tuna processing by-products (mainly dark muscle) into valuable foods. the characteristics of tu...

Temperature-induced changes in protein intrinsic fluorescence of native, delipidated and deionized purple membranes are investigated. It is found that the removal of cations most strongly affects the protein and its thermal stability. The denaturation of dei-BR completes at 70 degrees C, while delipidated and native BR still maintain their native structure at this temperature. Both the quantum ...

Using Agrobacterium, we developed a method to transform an Arabidopsis cell suspension culture. A stably transformed cell line expressing high levels of firefly luciferase (Luc) was used for in vivo studies of thermal denaturation and renaturation of the enzyme and the protective role of different chaperones. Luc activity was monitored under heat stress and recovery conditions in control, therm...

Kenaf seed meal protein concentrate (KSPC) and lyophilized kenaf milky extract (KSMEPC) were obtained via alkaline extraction. The proximate compositions, amino acids functional properties of both concentrates compared. KSPC was found to be significantly higher in protein, essential non-essential but, lower lipid carbohydrate contents than KSMEPC. solubility, water absorption capacity denaturat...

Human serum albumin has been used as a model protein for protein folding and ligand binding studies over many decades. Due to its long life period and high concentration in plasma, HSA is highly sensitive to glycation. It is reported that 175 mg/dL glucose concentration is a threshold of kidney activity for the beginning of excretion of glucose. pH denaturation of HSA in absence and presence of...

Transfer of an aromatic chromophore from the interior of a protein into the water solvent upon denaturation of the protein produces an absorption change throughout the ultraviolet region, 215 to 320 rnp, approximately 6 times the magnitude of that produced by transfer of the same chromophore from 20% ethylene glycol into water. In the 230 rnE.1 wave length region, the transfer of an indole chro...