Escherichia coli K-12 colicinogenic for ColEl yielded mutants that appeared to produce colicin at 43 C but not at 30 or 37. These mutants proved to have the mutation recA Further study revealed that both recA and recA + bacteria, when carrying ColE1 or ColE2, produce more colicin during growth at higher temperatures or after brief exposure to temperatures beyond the growth range. Counts of lacu...

In this review, we analyse and discuss 60+ individual cysteine protecting groups reported over the past several decades, highlighting their applications in peptide synthesis protein science.

Mixed and collagen protein synthesis is elevated for as many as 3 days following exercise. Immediately after exercise, enhanced amino acid availability increases synthesis of mixed muscle protein, but not muscle collagen protein. However, the potential for synergic effects of amino acid ingestion with exercise on both mixed and collagen protein synthesis remains unclear. We investigated muscle ...

The branched chain amino acids (BCAAs) are leucine, valine and isoleucine. A multi-million dollar industry of nutritional supplements has grown around the concept that dietary supplements of BCAAs alone produce an anabolic response in humans driven by a stimulation of muscle protein synthesis. In this brief review the theoretical and empirical bases for that claim are discussed. Theoretically, ...

keeping constant all agronomic factors except temperature is a way to follow heat shock proteins (hsps) expression. three different sunflower hybrids derived from the cross between an inbred line, cms ha 89, and three different restorer inbred lines, with resistance, susceptible, and normal reactions to drought, were produced. in order to investigate the impact of temperature on protein accumul...

Yuan CL, Sharma N, Gilge DA, Stanley WC, Li Y, Hatzoglou M, Previs SF. Preserved protein synthesis in the heart in response to acute fasting and chronic food restriction despite reductions in liver and skeletal muscle. Am J Physiol Endocrinol Metab 295: E216–E222, 2008. First published April 29, 2008; doi:10.1152/ajpendo.00545.2007.—Whole body protein synthesis is reduced during the fed-to-fast...

We measured rates of protein synthesis in vivo in subcellular fractions (soluble, myofibrillar and stromal fractions) of the heart and the gastrocnemius from rats after fasting or under hypoxic conditions (i.e. atmospheres containing 5% or 10% O2). Such interventions are known to inhibit protein synthesis under some circumstances. The recovery of tissue protein after fractionation was 80-100%. ...

We demonstrate that NGF couples the rate of degradation of long-lived proteins in sympathetic neurons to the rate of protein synthesis. Inhibiting protein synthesis rate by a specific percentage caused an almost equivalent percentage reduction in the degradation rate of long-lived proteins, indicating nearly 1:1 coupling between the two processes. The rate of degradation of short-lived proteins...

It is shown that in loach embryos the incorporation of precursors into protein takes place in the blastoderm cells only. The change of the rate of incorporation of labelled amino acids into protein of the blastoderm separated from the yolk at successive developmental stages reflects the changes in the level of protein synthesis in intact embryos of the same developmental stages. Typical periodi...

It is shown that in loach embryos the incorporation of precursors into protein takes place in the blastoderm cells only. The change of the rate of incorporation of labelled amino acids into protein of the blastoderm separated from the yolk at successive developmental stages reflects the changes in the level of protein synthesis in intact embryos of the same developmental stages. Typical periodi...