A technique to isolate DNA from deer meat was established authenticate the species and successfully extracted meat. The method based on Epicentre MasterPureTM Complete RNA Purification Kit extraction kit with slight modification by increasing volume of Proteinase K RNase 5 µL 20 each. recovery then analysed Polymerase Chain Reaction using cytochrome b gene oligonucleotide primers for family, wh...

Actin bound to the adenovirus proteinase (AVP) with a lower equilibrium dissociation constant, 4.2 nM, than those exhibited by two viral, nuclear cofactors for AVP, the 11-amino acid peptide pVIc and the viral DNA. The k(cat)/K(m) ratio for substrate hydrolysis by AVP increased 150,000-fold in the presence of actin. The 11-amino acid residue peptide corresponding to the C-terminus of actin, whi...

The bovine serum albumin (BSA) protein is employed in genosensors as nonspecific binding blocker. In this work, the influence of BSA on electrochemical response a DNA-based biosensor explored by impedance spectroscopy (EIS) and double pulse voltammetry technique. Each buffer component hybridization studied bare gold electrodes single-stranded DNA (ssDNA)-functionalized electrodes. found to have...

This study include isolation of some types Aspergillus sp from different sources such as ( soil, vegetables), three appeared; fumegates , nidulans and ochracaeus ,then  tested the virulence factors these fungi production enzymes (proteinase ,phospholipase ,heamolycin ,lipase urease ) give positive result for of  phospholipase  ,lipase urease; enzyme proteinase product only A. .al...

Western blotting (immunoblotting) of Cowdria ruminantium antigens with goat or mouse antiserum identified a periodate-resistant, proteinase K-sensitive immunodominant antigen of 32,000 daltons. This protein, designated Cr32, could be demonstrated in goat choroid plexus infected with one of two different Cowdria stocks. Antisera against nine different Cowdria stocks from Africa and the Caribbean...

Differential sensitivity for the release of PCR-detectable genomic DNA upon boiling in water is reported for 45 Campylobacter jejuni and Campylobacter coli strains isolated in Egypt. All of the strains released PCR-detectable DNA when treated with proteinase K and sodium dodecyl sulfate. When DNA was extracted from these strains by boiling in water, nine (20%) of the strains were PCR negative o...

Activity of the lactococcal cell envelope-located serine proteinase depends on the presence of membrane-associated lipoprotein PrtM. To differentiate between the action of the proteinase and the action of PrtM in the process of proteinase maturation, an inactive form of the lactococcal proteinase was constructed. This was done by mutating one of the three amino acids thought to constitute the a...