Proteomics involves the identification of unknown proteins following their separation, often using two-dimensional electrophoresis, digestion of particular proteins of interest by trypsin, determination of the molecular weight of the resulting peptides, and database searching to make the identification of the proteins. Application of proteomics to Alzheimer's disease (AD), the major dementing d...

Plant pathogenic fungi cause important yield losses in crops. In order to develop efficient and environment-friendly crop protection strategies, molecular studies of the fungal biological cycle and interaction with its host are necessary. For that reason, several approaches have been made using both classical genetic, cell biology and biochemistry and modern, holistic and high-throughput, omic ...

Denaturing Isoelectric Focusing Free-Flow Electrophoresis (IEF-FFE) Fractionation of Crude Protein Mixtures Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn This protocol was adapted from "Denaturing IEF-FFE Fractionation of Crude Protein Mixtures," contributed by Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn, Chapter 19, in Purifying Proteins for Proteomics (ed. Simpson). ...

Proteomics is a new methodological and conceptual approach to the study of live systems, which aims to map the whole proteom - the protein complement of genome. It aspires to describe quantitatively and qualitatively all proteins present at the given moment in the cell, tissue or the organism. The principal tools of proteomics are separation techniques based on electrophoresis and chromatograph...

The most functional compartment encoded by the genome is proteome. Therefore study of proteome i.e. proteomics is the promising approach in identification, separation and quantitation of functional changes. It aims to gain a comprehensive understanding of the expressions, modifications, interactions, and regulation of proteins in cells. The power of two-dimensional electrophoresis and advances ...

The class of tensor product cubic splines is considered in an optimization process for two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) image alignment problems arising in proteomics studies. Numerical results are presented along with comparisons with previous results based on piecewise affine and bilinear transformations.

In the nowadays mainly mass spectrometry-driven world of Proteomics, one can frequently read and hear that 2D gel-based electrophoresis methods are no longer used for proteome analysis. This short review is a compilation of facts why the gel-based workflow is not dead.

Proteomics relies on the separation of complex protein mixtures using bidimensional electrophoresis. This approach is largely used to detect the expression variations of proteins prepared from two or more samples. Recently, attention was drawn on the reliability of the results published in literature. Among the critical points identified were experimental design, differential analysis and the p...

Functional genomics (transcriptomics and proteomics) is a global, systematic and comprehensive approach to identification and description of the processes and pathways involved in the normal and abnormal physiological states. The most applied methods of functional genomics today are DNA microarrays and proteomics methods, primarily twodimensional gel electrophoresis coupled with mass spectromet...

The proteome is defined as the protein complement to the genome. Proteomics is the study of the proteome. Several techniques are frequently used in proteomics; these include 2-hybrid systems, 2-dimensional gel electrophoresis, and mass spectrometry. Systems biology is a scientific approach that takes into account the complex relationships among and between genes and proteins and determines how ...