Desiccation tolerance of protoplasts isolated from germinating pea (Pisum sativum L. cv. 'Alaska') embryonic axes depends, in part, on the osmotic strength and composition of the suspending medium. To determine the reason for this dependence and whether treatment with different solutions results in different types of damage, protoplast recovery and survival were assessed after dehydration to a ...

Abstract Natural rubber is an important industrial raw material and commercially produced by trees (Hevea brasiliensis). The sucrose transporter HbSUT3 plays essential role in production. Its expression latex (cytoplasm of rubber-producing laticifers) induced bark treatment with Ethrel, ethylene releaser, the inducing effect correlates well Ethrel-stimulated yield increase. However, mechanisms ...

The present study reports an efficient protocol for isolation and regeneration of protoplasts from callus of Fritillaria imperialis L. There is no published method recommended for protoplast isolation and regeneration from Fritillaria imperialis L. A range of factors, which influence the success of isolation and regeneration of F. imperialis protoplasts, were investigated. From the results obta...

The breeding of triploid banana cultivars with improved traits, such as yield and disease resistance, remains a major challenge for breeders. One reason is that the molecular study functional gene analysis in bananas fall behind due to difficulties its genetic manipulation. plant protoplast-based transient transformation has been documented widely used versatile convenient system many species. ...