The electrophoretic mobility shift assay (EMSA), or gel mobility shift assay, is a popular and powerful technique for the detection of RNA-protein interactions. It relies on the fact that naked RNA has certain mobility on nondenaturing gels, but if the RNA is bound by protein, the mobility of the RNA is reduced. Therefore, the binding of protein results in a characteristic upward shift of the R...

The objective of this study was to identify the symptoms related to the overall health status associated with shift work in nursing and relate it to the quality of sleep. The study was performed at Hospital da Irmandade da Santa Casa in Poços de Caldas, Minas Gerais State. The participants were 136 nursing professionals, of an average age of 33.1 years, divided into the following categories: nu...

Maximal length Feedback with Carry Shift Register sequences have several remarkable statistical properties. Among them is the property that the arithmetic correlations between any two cyclically distinct decimations are precisely zero. It is open, however, whether all such pairs of decimations are indeed cyclically distinct. In this paper we show that the set of distinct decimations is large an...

Anewsoftware-baseddtestvectorcompressionn technique is proposedd for usingg ann embeddedd processor too test the other components of aa system-on-a-chipp (SOC). The tester transfers compressedd test dataa too the processor's on-chipp memory, andd the processor executes aa small program whichh decompresses the dataa andd applies it too the scann chains of eachh core-under-test. The proposedd dec...

A novel algorithmic approach to the synthesis of fairly long DNA molecules with nonrecurring sequences is demonstrated. The scheme exploits chemical embodiment of shift registers (SR) to execute algorithms similar to those used to generate pseudorandom numbers on a computer. Single stranded DNA molecules guide the synthesis of double stranded DNA according to the SR truth table. The SR logic fa...

An algorithm is given for the reconstruction of the initial state of a key–stream generator (KSG) consisting of a short linear feedback shift register (length ≤ 30), whose clock is controlled by an algebraically simple internal KSG. The algorithm is based on the fact that the expected number of possible LFSR initial states exponentially decreases with the length of the known part of the output ...

BACKGROUND Shift work has been associated with occupational stress in health providers and in those working in some industrial companies. The association is not well established in the law enforcement workforce. Our objective was to examine the association between shift work and police work-related stress. METHODS The number of stressful events that occurred in the previous month and year was...

Pulsed Fourier transform proton magnetic resonance spectroscopy was used to study the glutamatealanine transaminase-catalyzed incorporation of deuterium from solvent deuterium oxide into the cy and p positions of L-alanine. It was found that the /3 proton resonance signal initially disappears slightly faster than the signal due to the LY proton, but whereas the o( proton signal decays exponenti...