نتایج جستجو برای: pseudomonas cepacia lipase

تعداد نتایج: 70309  

Journal: :Antimicrobial agents and chemotherapy 2009
Laurent Poirel José-Manuel Rodriguez-Martinez Patrick Plésiat Patrice Nordmann

Chromosomally encoded ss-lactamases from the Burkholderia cepacia complex species (formerly Pseudomonas cepacia) were characterized. Cloning and sequencing identified an Ambler class A ss-lactamase (PenB) from B. cenocepacia. It shares 82% amino acid identity with the PenA ss-lactamases previously identified from B. multivorans 249. Its expression was dependent upon a LysR-type regulatory prote...

Journal: :Journal of the Japanese Association for Infectious Diseases 1983

Journal: :Memórias do Instituto Oswaldo Cruz 1993

Pseudomonas aeruginosa BBRC-10036 was used for lipase production. The organism secreted the enzyme extracellulary. In order to purify the enzyme, precipitation was done first, and then this lipase has been purified by Ion exchange Chromatography leading to 2.3-fold purification and 11.47% recovery. Lipase from P.aeruginosa was entrapped into Ca-alginate gel beads and effect of independent varia...

Journal: :Journal of the Japanese Association for Infectious Diseases 1980

Journal: :Protein engineering 1994
O Misset G Gerritse K E Jaeger U Winkler C Colson K Schanck E Lesuisse V Dartois M Blaauw S Ransac

Within the BRIDGE T-project on lipases we investigate the structure-function relationships of the lipases from Bacillus subtilis and Pseudomonas aeruginosa. Construction of an overproducing Bacillus strain allowed the purification of > 100 mg lipase from 30 l culture supernatant. After testing a large variety of crystallization conditions, the Bacillus lipase gave crystals of reasonable quality...

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