Campania's buffalo mozzarella is a greatly appreciated cheese in Italy and worldwide. From a microbiological standpoint, it is a highly perishable food and potentially at risk of contamination by pathogens such as Listeria monocytogenes (L. monocytogenes). The present paper reports the results of a challenge test carried out to assess the population dynamics of L. monocytogenes, alone and in pr...

Pseudomonas fluorescens strains that produce the polyketide antibiotic 2,4-diacetylphloroglucinol (2,4-DAPG) are among the most effective rhizobacteria that suppress root and crown rots, wilts, and damping-off diseases of a variety of crops, and they play a key role in the natural suppressiveness of some soils to certain soilborne pathogens. Root colonization by 2,4-DAPG-producing P. fluorescen...

A chromosomal locus encoding biosynthetic genes for a putative peptide synthetase of Pseudomonas fluorescens was identified and cloned. To achieve this, two sets of degenerated oligonucleotide primers KAGGA:SGTTG and TGD:LGG were used in PCR. These primers were selected based on highly conserved units of known peptide synthetases involved in adenylation and thiolation regions of Bacillus subtil...

Fusarium wilt diseases caused by the fungus Fusarium oxysporum lead to significant yield losses of crops. Biocontrol agents such asTrichoderma viride , Trichoderma harzianum and Pseudomonas fluorescens are applied to control Fusarium wilt of Arachis hypogaea. Experiments were conducted on the effect of culture filtrates of T.viride (1%), T.harzianum (1.5%), and P. fluorescens (2%) on biochemica...

to study of biological control of charcoal rot of melon caused by macrophomina phaseolina, 187 isolates of fluorescens pseudomonas isolated of rhizosphere and soil of melon plants. then 12 isolates belong to i, iii and v biovars of p. fluorescens and b biovar of p. putida were selected for greenhouse experiments based on inhibition amount. pseudomonas isolates inhibited mycelial growth signific...

Glucose oxidation by Pseudomonas Jluorescens (1) and Pseudomonas aeruginosa (2) has been shown to proceed via gluconate to 2-ketogluconate without the involvement of phosphorylated intermediates. 2-Ketogluconate is oxidized further in growing cultures (3) and resting cell suspensions (4), but attempts to obtain dried cell preparations or cell-free extracts which oxidize 2-ketogluconate have bee...

Rapid increase of resistance to antibacterial agents in recent years has become a major global public health problem.1,2) The development of antibiotics with novel mechanisms of action is one of the most important measures leading to the control of bacterial infectious diseases in the new millennium. The genomic antibacterial approach plays a critical role in the discovery of new targets by sea...

In many microorganisms the first step for alkane degradation is the terminal oxidation of the molecule by an alkane hydroxylase. We report the characterization of a gene coding for an alkane hydroxylase in a Burkholderia cepacia strain isolated from an oil-contaminated site. The protein encoded showed similarity to other known or predicted bacterial alkane hydroxylases, although it clustered on...