The long-patch mismatch repair pathway contributes to the cytotoxic effect of methylating agents and loss of this pathway confers tolerance to DNA methylation damage. Two methylation-tolerant mouse cell lines were identified and were shown to be defective in the MSH2 protein by in vitro mismatch repair assay. A normal copy of the human MSH2 gene, introduced by transfer of human chromosome 2, re...

Thermal conductivity of graphite bipolar plates (BPP) decreases with temperature. Thermal Contact resistance (TCR) between BPP and GDLs decreases with compression. GDL-BPP TCR increases with MPL and PTFE, regardless of the PTFE loading. High PTFE loading, MPL, and BPP outof-flatness increase the TCR dramatically. The graphite BPP-GDL TCR is a dominant resistance in a BPP-GDL assembly. a r t i c...

PDMS microfluidic devices whose whole inner surfaces are covered with PTFE (polytetrafluoroethylene) are described. The major problems of PDMS fluidic devices in practical use, are surface degradation with organic solvents and unexpected absorption of chemical materials. In order to overcome these problems, spin/dip-coated perfluoro amorphous polymer is used to form the passivation PTFE layer o...

Mitral valve (MV) repair is indicated for patients with severe MR. We report a case of acute MR caused by patch detachment after posterior leaflet augmentation in MV repair. A 65-year-old male underwent MV repair with posterior leaflet augmentation and coronary artery bypass graft 1 month prior to this study. An inverted T-shaped incision was made on the posterior mitral leaflet (PML), and a pi...

Two different greases formulated using MoS2 and a combination of ZDDP and functionalized PTFE (F-PTFE) were examined under spectrum loading conditions where loads, frequency, and duration of the steps were treated as variables. Combination of ZDDP and F-PTFE were synergistic resulting in a significant reduction in the wear and friction under spectrum loading condition. Decreasing the time step ...

BACKGROUND Although the "open abdomen" has likely contributed to improved outcomes in trauma patients, the challenge of subsequent fascial closure has emerged. Since mid 2004, we have incorporated Wittmann Patch staged abdominal closure into our management of the open abdomen. The purpose of this study was to evaluate the impact of this device on our incidence of fascial closure versus planned ...

BACKGROUND Open mesh used in anterior inguinal hernia repair can be configured as a flat patch (Lichtenstein operation) or as a cone-shaped preformed plug and supplementary patch (plug-and-patch operation; Perfix Plug; Davol Inc, Cranston, RI). METHODS One hundred forty-one patients were randomly allocated and blinded to receive either a Lichtenstein patch or a Perfix plug-and-patch. Informat...

Introduction: The effects of different solid substrates including: carbon nanofibers (CNFs), activated carbon, alumina, silica, and poly vinyl pyrolidone (PVP) were compared for high pressure synthesis of polytetrafluoroethylene (PTFE, (CF2)n) nanoparticles via the adsorption of thermally synthesized tetrafluoroethylene (TFE, C2F4) as monomer. Aim: Syntheses and characterization PTFE nanopartic...

OBJECTIVES Laparoscopic repair of incisional ventral hernias with ePTFE mesh continues to evolve, with variable reporting of surgical techniques and outcomes. This report of 34 cases discusses, with a literature review of laparoscopic incisional hernia repair, specific factors associated with three recurrences. METHOD Retrospective analysis and review of the literature. RESULTS Thirty-two p...