نتایج جستجو برای: ras mutations

تعداد نتایج: 198454  

2015
Irena Antic Marco Biancucci Yueming Zhu David R. Gius Karla J. F. Satchell

Ras (Rat sarcoma) protein is a central regulator of cell growth and proliferation. Mutations in the RAS gene are known to occur in human cancers and have been shown to contribute to carcinogenesis. In this study, we show that the multifunctional-autoprocessing repeats-in-toxin (MARTX) toxin-effector domain DUF5(Vv) from Vibrio vulnificus to be a site-specific endopeptidase that cleaves within t...

Journal: :Blood 1989
P C Cogswell R Morgan M Dunn A Neubauer P Nelson N K Poland-Johnston A A Sandberg E Liu

Seventy cases of chronic myelogenous leukemia (CML) were analyzed for the presence of ras mutations using polymerase chain reaction (PCR), oligonucleotide hybridization, and direct PCR sequencing. All cases had preceding cytogenetic and bcr rearrangement studies. Aberrant ras genes were detected in none of 39 patients with Philadelphia (Ph) chromosome or bcr/abl rearrangement positive chronic-p...

2016
Hyeran Sung Krishna L. Kanchi Xue Wang Kristen S. Hill Jane L. Messina Ji-Hyun Lee Youngchul Kim Nathan D. Dees Li Ding Jamie K. Teer Shengyu Yang Amod A. Sarnaik Vernon K. Sondak James J. Mulé Richard K. Wilson Jeffrey S. Weber Minjung Kim

Inactivation of Ras GTPase activating proteins (RasGAPs) can activate Ras, increasing the risk for tumor development. Utilizing a melanoma whole genome sequencing (WGS) data from 13 patients, we identified two novel, clustered somatic missense mutations (Y472H and L481F) in RASA1 (RAS p21 protein activator 1, also called p120RasGAP). We have shown that wild type RASA1, but not identified mutant...

Journal: :International journal of oncology 2007
Apostolos Zaravinos John Bizakis Giannoula Soufla George Sourvinos Demetrios A Spandidos

Although it is well established that ras genes contribute to tumourigenesis either through the accumulation of mutations or by aberrant expression in a wide range of human cancers, little is known regarding their involvement in human nasal polyps (NPs). In the present study, the occurrence of mutations in codons 11 and 12 of the ras family genes was examined by PCR/RFLP and direct sequencing in...

Journal: :Annals of oncology : official journal of the European Society for Medical Oncology 1999
H Mulcahy M J Farthing

Chromosomal abnormalities, including mutations, deletions and allelic losses of different oncogenes and tumour suppressor genes have been discovered in the DNA of cancer cells and the application of molecular biological techniques now permits identification of these alterations in tumours. Although it has been possible to detect potentially important genetic alterations in tumour material for s...

Journal: :Cancer research 1990
T Victor R Du Toit A M Jordaan A J Bester P D van Helden

The molecular mechanisms underlying the induction of esophageal and gastric cancer are not yet understood. It is possible that different etiological factors from geographically distinct areas play a role in the onset of these cancers. Twenty-seven primary esophageal and 11 gastric cancers originating from the high-incidence areas of South Africa were analyzed for the presence of ras protooncoge...

Journal: :Clinical cancer research : an official journal of the American Association for Cancer Research 1997
F M Scott R Modali T A Lehman M Seddon K Kelly E C Dempsey V Wilson M S Tockman J L Mulshine

A high frequency of K-ras mutations may indicate preneoplastic changes in the bronchial epithelium as a result of genotoxic injury. With the use of sensitive detection techniques, we report a higher prevalence of K-ras mutations in bronchoalveolar lavage than has been reported previously for lung cancer. A PCR/ligase chain reaction technique was used to determine K-ras codon 12 mutations in a g...

2006
Johannes L. Bos

Mutations in codon 12, 13, or 61 of one of the three ras genes, 11-ra.v, k-ra.v, and N-ra.v,convert these genes into active oncogenes. Rapid assays for the detection of these point mutations have been developed recently and used to investigate the role mutated ras genes play in the pathogenesis of human tumors. It appeared that ras gene mutations can be found in a variety of tumor types, althou...

2006
Johannes L. Bos

Mutations in codon 12, 13, or 61 of one of the three ras genes, 11-ra.v, k-ra.v, and N-ra.v,convert these genes into active oncogenes. Rapid assays for the detection of these point mutations have been developed recently and used to investigate the role mutated ras genes play in the pathogenesis of human tumors. It appeared that ras gene mutations can be found in a variety of tumor types, althou...

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