The species diversity of freshwater crabs Bangladesh is poorly known. In this study, Acanthopotamon fungosum (Alcock, 1909) and Maydelliathelphusa edentula are reported as new records from Bangladesh. two were identified through morphology molecular phylogeny based on 16S rDNA gene sequences. Herein, diagnostic characters both provided respective to close congeners. There concern over the conse...

BACKGROUND Genetic modification is a prerequisite to realizing the full potential of human embryonic stem cells (hESCs) in human genetic research and regenerative medicine. Unfortunately, the random integration methods that have been the primary techniques used keep creating problems, and the primary alternative method, gene targeting, has been effective in manipulating mouse embryonic stem cel...

Methods that enabled the identification, detection, and enumeration of Bifidobacterium species by PCR targeting the transaldolase gene were tested. Bifidobacterial species isolated from the feces of human adults and babies were identified by PCR amplification of a 301-bp transaldolase gene sequence and comparison of the relative migrations of the DNA fragments in denaturing gradient gel electro...

A new species of quill mites Picobia dziabaszewskii sp. nov. (Acari, Syringophilidae) parasitizing Garrulax formosus (Verreaux) (Passeriformes: Leiothrichidae) is described using combined data sources: standard morphological information extended by mitochondrial cytochrome oxidase I sequence (COI) and domene D2 of the nuclear gene 28S rDNA data (DNA barcode). Additionally, the case of polymorph...

Endophytic bacterial diversity was estimated in Mexican husk tomato plant roots by amplified rDNA restriction analysis and sequence homology comparison of the 16S rDNA genes. Sixteen operational taxonomic units from the 16S rDNA root library were identified based on sequence analysis, including the classes Gammaproteobacteria, Betaproteobacteria, Actinobacteria, and Bacilli. The predominant gen...

The objective of this study was to determine whether different isolates of Babesia microti could be distinguished from morphologically similar isolates of B. gibsoni by using a ribosomal DNA (rDNA) probe. A Babesia-specific rDNA probe was obtained by polymerase chain reaction amplification of sequences from B. microti DNA using universal primers directed against highly conserved portions of the...

Infections by ectoparasitic trichodinid ciliates cause epidermal damages to artificially reared juvenile barfin flounder Verasper moseri of mariculture fisheries in Hokkaido, northern Japan. ​In the present study, morphological observation and molecular phylogenetic analysis using small subunit ribosomal RNA gene (SSU rDNA) were performed identify ciliates. ​Morphological denticles adoral cilia...

The genetic diversity and identification of slow- and fast-growing soybean root nodule bacterial isolates from different agro-climatic regions in Mpumalanga, Limpopo and Gauteng Provinces of South Africa were evaluated. The 16S-rDNA-RFLP analysis of 100 rhizobial isolates and eight reference type strains placed the isolates into six major clusters, and revealed their site-dependent genomic dive...

In June 2020, many plants exhibited symptoms of root rot and foliar blight in the experimental field common sage Şanlıurfa province, Turkey. The pathogen was identified as Neoscytalidium novaehollandiae based on morphological characteristics phylogenetic analysis partial sequence transcription elongation factor 1-α gene internal transcribed spacer rDNA. Koch’s postulates were fulfilled by succe...

The ribosomal RNA gene (rDNA) repeats form a historically well-researched region in the chromosome. Their highly repetitive structure can be identified easily which has enabled studies on DNA replication, recombination, and transcription. The region is one of the most unstable regions in the genome because of deleterious recombination among the repeats. The ribosomal RNA gene repeats use a uniq...