Maleimides remain the reagents of choice for the preparation of therapeutic and imaging protein conjugates despite the known instability of the resulting products that undergo thiol-exchange reactions in vivo. Here we present the rational design of carbonylacrylic reagents for chemoselective cysteine bioconjugation. These reagents undergo rapid thiol Michael-addition under biocompatible conditi...

Trudinger, P. A. (Division of Plant Industry, Canberra, Australia). Effect of thiol-binding reagents on the metabolism of thiosulfate and tetrathionate by Thiobacillus neapolitanus. J. Bacteriol. 89:617-625. 1965.-Iodoacetamide, N-ethyl maleimide (NEM), p-chloromercuribenzoate (CMB), Mercurochrome, and HgCl(2) inhibited the oxidation of thiosulfate to sulfate by Thiobacillus neapolitanus; tetra...

Targeted genome editing has become a powerful genetic tool for studying gene function or for modifying genomes by correcting defective genes or introducing genes. A variety of reagents have been developed in recent years that can generate targeted double-stranded DNA cuts which can be repaired by the error-prone, non-homologous end joining repair system or via the homologous recombination-based...

Several methods have been reported for preparation of nitriles by dehydration of aldoximes using selenium dioxide-chloroform, diphosphorus tetraiodide, trifluoroacetic anhydride-pyridine, chlorosulphonyl isocyanate, 4,6-dipheny-2-methylthio-pyriliumtetrafluoro borate, copper (II) acetate-acetonitrile, trichloroacetyl chloride-triethylamine, triphenylphosphine/carbontetrachloride (PPh3/CCl4) in ...

Shotgun proteomic methods involving isobaric tagging of peptides enable high-throughput proteomic analysis. iTRAQ reagents allow simultaneous identification and quantitation of proteins in four different samples using tandem mass spectrometry (MS). In this article, we provide a brief description of proteome analysis using iTRAQ reagents and review the current applications of these reagents in p...

Custom-made pencils containing reagents dispersed in a solid matrix were developed to enable rapid and solvent-free deposition of reagents onto membrane-based fluidic devices. The technique is as simple as drawing with the reagent pencils on a device. When aqueous samples are added to the device, the reagents dissolve from the pencil matrix and become available to react with analytes in the sam...

This study compared different commercially available viability reagents. The growth indicator reagents include p-iodonitrotetrazolium violet (INT), PrestoBlue, and Alamar Blue which were used for antimicrobial analysis against Streptococcus mutans, Prevotella intermedia, Propionibacterium acnes, and Mycobacterium tuberculosis. PrestoBlue and Alamar Blue are resazurin based reagents that resulte...

The article presents one of the new approaches to theoretical assessment collecting ability reagents. efficiency reagents-collectors with different chemical composition used for flotation coking coals was studied. A comparative activity kerosene, mineral oil, thermal gas KETGOL and FLOTEK is given. criteria above coal sludge were specified. correlation established between indicators by reagents...

According to the unique properties and many applications of nano gold powder, it was prepared from an acid leaching tail solution. The low value residual gold ions existed in acid leaching tail solution in Mouteh gold mine in Iran with fellow heavy metals as pollutants (Fe2+, Cu2+, and Zn2+). Preparation of nano gold particles is achieved by two steps: First, by addition of ammonia as separatio...