Journal:
:iranian journal of parasitology
0
somayeh ghotloo dept. of immunology, faculty of medicine, shahid beheshti university of medical sciences, tehran, iran
mostafa haji mollahoseini dept. of immunology, faculty of medicine, shahid beheshti university of medical sciences, tehran, iran and dept of applied cell sciences, school of advance technologies in medicine, shahid beheshti university of medical sciences, tehran, iran
ali najafi dept. of immunology, pasteur institute of iran, tehran, iran
farshid yeganeh dept. of immunology, pasteur institute of iran, tehran, iran
background: limiting dilution assay is considered as the gold standard method for quantifying the number of parasites in the animal model of leishmania infection. nowadays, real-time pcr is being increasingly applied to quantify infectious agents. in the present study , a real-time pcr assay was developed to estimate parasite burdens in lymph nodes of leishmania major infected balb/c mice. enu...
