Gene expression analysis at the single-cell level is critical to understanding variations among cells in heterogeneous populations. Microfluidic reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) is well suited to gene expression assays of single cells. We present a microfluidic approach that integrates all functional steps for RT-qPCR of a single cell, including i...

Rhizoctonia solani anastomosis group AG2-2 IIIB is a severe sugar beet and maize pathogen. It causes crown and root rot disease which le ads to y ield losses world-wide. The soil-borne pathogen is d ifficult to detec t and quantify by conventional methods. We developed a real-time PCR (qPCR) assay for the quantification of genomic DNA of Rhizoctonia solani AG2-2 IIIB based on the ITS region of ...

Bioaerosol detection in real time is an urgent civilian and military requirement. In this article, bioaerosol mass spectrometry, an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence, real-time qPCR, and FCM/FL were discussed. Although, challenging work remains to determine the interfering substances (e.g. par...

In recent years, increasing numbers of human campylobacteriosis cases caused by contaminated water have been reported. As the culture-based detection of Campylobacter is time consuming and can yield false-negative results, the suitability of a quantitative real-time PCR method in combination with an ethidium monoazide pretreatment of samples (EMA-qPCR) for the rapid, quantitative detection of v...

UNLABELLED The qpcR library is an add-on to the free R statistical environment performing sigmoidal model selection in real-time quantitative polymerase chain reaction (PCR) data analysis. Additionally, the package implements the most commonly used algorithms for real-time PCR data analysis and is capable of extensive statistical comparison for the selection and evaluation of the different mode...

Arcelin, the antimetabolic protein from wild pulses is a known natural insecticidal molecule. Wild pulses with high arcelin content could serve as potential source to. increase the levels of insect resistance in cultivated pulse crops. In this study, arcelin (Arl) gene expression was screened in seven stored product insect pest resistant wild pulse varieties using real time RT-qPCR. Arcelin gen...

To gain insight into the mechanism of action of MYCN and TP53 genes in neuroblastoma pathogenesis, and to create a model system for future exploration of targeted therapeutics, we decided to exploit RNAi as an experimental tool for suppressing the expression of MYCN and TP53. The MYCN gene was transiently suppressed using several 27-mer siLentMerTM* Dicer-substrate small interfering RNA (siRNA)...

Quantitative real-time PCR (QPCR) has emerged as an accurate and valuable tool in profiling gene expression levels. One of its many advantages is a lower detection limit compared to other methods of gene expression profiling while using smaller amounts of input for each assay. Automated qPCR setup has improved this field by allowing for greater reproducibility. Its convenient and rapid setup al...

Citation for the published paper: Philippe Blanchard, Sylvain Guillot, Karina Antùnez, Hemma Köglberger, Per Kryger, Joachim R. de Miranda, Stéphanie Franco, Marie-Pierre Chauzat, Richard Thiéry, Magali Ribière. (2014). Development and validation of a real-time twostep RT-qPCR TaqMan (R) assay for quantitation of Sacbrood virus (SBV) and its application to a field survey of symptomatic honey be...