Background: Newcastle disease is a major avian disease that causes enormous economic loss in poultry industry. There have been a number of reports on the suitability of plant-based recombinant vaccine against this disease. Fusion (F) and hemagglutinin-neuraminidase (HN) epitopes of the Newcastle disease virus (NDV) represent the major immunogenic sites for development of recomb...

Background: Rabies virus (RABV) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. L protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication. Objectives: A truncated L protein of Rabies virus is being cloned, expressed and purified to produce relevant...

results cloning and subcloning of the flic gene were confirmed by colony pcr, restriction enzymes digestion and dna sequencing of the recombinant plasmids pprime-flic and pvax-flic. the expression of flagellin protein in eukaryotic cells was approved by immunofluorescence assay (ifa), western blotting analysis and the reverse transcriptase polymerase chain reaction (rt-pcr) method. conclusions t...

Objective(s): Hepatitis C is a major cause of liver failure worldwide. Current therapies applied for this disease are not fully effective and produce side effects in most cases. Non-structural protein 3 helicase (NS3) of HCV is one of the key enzymes in viral replication and infection. Therefore, this region is a promising target to design new drugs and therapies against HCV infection. The aim ...

expression of htlv-i p19 protein in an escherichia coli expression system always leads to the formation of inclusion body. solubilisation and refolding of the inclusion bodies is complex, time consuming and difficult during large-scale preparation. this study aimed to express and purify a soluble form of recombinant htlv-i p19 protein in an e. coli expression system. the synthetic dna encoding ...

objectives: in present study we aimed to clone the lux i gene encoding n-acyl-homoserine synthase detected in biofilm forming clinical isolates of acinetobacter baumannii and study its expression in escherichia coli transformants. materials and methods: four a. baumannii hospital strains which demonstrated strong biofilm activity were selected in this investigation. the presence of lux i gene w...

background: the purpose of this study was to clone, express, and purify a novel multidomain fusion protein of micobacterium tuberculosis (mtb) in a prokaryotic system. methods: an hspx/esxs gene construct was synthesized and ligated into a pgh plasmid, e. coli top10 cells were transformed, and the vector was purified. the vector containing the construct and pet-21b (+) plasmid were digested wit...

background and purpose: brain-derived neurotrophic factor (bdnf) is present in the hippocampus and cerebral cortex in high concentrations and has an important role in development, maintenance and regeneration of the nervous system. the aim of this study was to produce active bdnf in e.coli using transforming plasmid. methods: to construct an expression plasmid of bdnf, a gene coding for it was ...

objective: development of high producing mammalian cell lines is a major bottleneck in manufacturing of recombinant therapeutic proteins. this study examines the effect of using the matrix attachment region from the human interferon beta gene in combination with promoter activation strategy with e1a 13s protein on human tissue plasminogen activator (t-pa) expression in chinese hamster ovary (ch...

alcanivorax borkumensis is a marine bacterium that has ability to grow on limited substrates that mainly is alkanes. the ability to use wide range of hydrocarbons is advantage of this bacterium to other marine community bacteria. a. borkumensis have two genetic systems for alkane biodegradation. the first system is alkane hydroxylase (alk-b1and alk-b2) and the second system is  cytochrome p450....