background: hepatitis c virus (hcv) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. therefore, designing new and effective therapeutics is of great importance. the aim of the current study was to construct a dna vaccine containing structural proteins of hcv and evaluation of its expression in a eukaryotic system. methods: struct...

background and aims: virus-like particles (vlps) have been suggested to be a promising recombinant vaccine approach. several studies have reported that the influenza vlps produced in insect cells is an effective vaccine candidate. due to crucial role of matrix m1 protein in assembly and budding of influenza particles, in all vlps structures, m1 protein have been considered as a main component. ...

Bacterial expression of eukaryotic proteins is a tool of ever-increasing importance in biochemistry and molecular biology. However, the majority of the recombinant eukaryotic proteins that have been expressed in bacteria are produced as fusion proteins and not in their native conformation. In particular, correct formation of quaternary structures by recombinant proteins in bacterial hosts has b...

Hepatitis B immunoglobulin (HBIG) is an effective treatment for hepatitis B, including postexposure prophylaxis of HBV infection, prevention reinfection in liver transplant patients, and reducing sexual transmission. This study investigated the synthesis human IgG antibodies that specifically target HBsAg subtype adr CHL-YN cells, a newly established cell line grows faster than CHO-K1. To achie...

ABSTRACT: Streptomyces sampsonii is a kind of biocontrol bacterium with antifungal effects, and chitinase one the main substances. To improve further study structure function gene S. sampsonii, we amplified target fragment by PCR, ligated to expression vector pET-32a, introduced resulting plasmid into Escherichia coli BL21 (DE3) induced chitinase. Then, recombinant was purified is-labelled prot...

Cell cycle related molecules in mammalian cochleae could provide a new avenue to restore hearing loss caused by a variety of genetic and environmental insults. CyclinA2 is one of the most important regulators of cell cycle, but its role in the mammalian cochlea is still unknown. So, it is necessary to construct an adenovirus vector carrying cyclinA2 gene for clarifying its function in the cochl...

conclusions our results showed that the recombinant protein produced by the pbad vector in the escherichia coli system was very efficient. background nontypeable haemophilus influenzae (nthi) is a significant pathogen in children, causing otitis media, sinusitis, conjunctivitis, pneumonia, and occasionally invasive infections. protein d (pd) belongs to the minor outer-membrane proteins of h. in...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

Background The use of Escherichia coli for the production of recombinant proteins is an established strategy to obtain biotechnological tools. However, the recombinant protein expression is dependent on temperature, bacterial culture time, induction period, nutrients, plasmid characteristics, and insert itself. Thus, the aim of this study was optimize the expression of recombinant gE protein (r...