When Escherichia coli Olll:B4 is exposed briefly to ethylenediaminetetraacetate (EDTA) it releases 30 to 50% of its lipopolysaccharide, determined not only by measuring percentage release of colitose, a sugar unique to this polymer, but also by measuring the amount of phenol-extractable lipopolysaccharide remaining with the cell. The fraction released cannot be significantly increased above 50%...

Eggs from the sea urchin, Lytechinus pictus, were injected with either EGTA or EDTA, and were subsequently fertilized. EGTA prevented cortical vesicle discharge and formation of the fertilization membrane. EDTA had either no effect, or sometimes retarded the elevation of the fertilization membrane, or reduced the percentage of eggs with elevated membranes. Theoretical considerations lead to est...

Current SQL aggregation functions have evident limitations for computing percentages, for which this paper proposes two SQL aggregation functions. The two novel aggregation functions are easy to use, which have wide applicability and can be efficiently evaluated. They may be used as a framework to study percentage queries and to generate efficient SQL code. Experiments compare our proposed perc...

AIMS To assess the reliability of percentage ideal weight for height (%WFH) as a measure in children. METHODS Forty two dietitians calculated %WFH of six children. Eleven of the 42 repeated the calculations. RESULTS Interexaminer estimates varied by 16.5 to 40 percentage points (mean 27.8). Intraexaminer variability was also large. CONCLUSIONS %WFH is an unreliable measure of nutritional ...

Studies of expressive speech have shown that discrete emotions such as anger, fear, joy, and sadness can be accurately communicated, also cross-culturally, and that each emotion is associated with reasonably specific acoustic characteristics [8]. However, most previous research has been conducted on acted emotions. These certainly have something in common with naturally occurring emotions but m...

The extracellular form of pullulanase (EC 3.2.1.41) from Klebsiella aerogenes has been purified to homogeneity by successive chromatography through diethylaminoethyl-cellulose, Sephadex G-200, and 1,6-diaminohexane-Sepharose. In addition, the cell-bound form of pullulanase has been released by the action of a serine endopeptidase obtained from Pronase and purified to apparent homogeneity. Prote...

PURPOSE To investigate the relationship between percentage of body fat and macular pigment (MP) optical density. METHODS One hundred healthy subjects of ages between 22 and 60 years volunteered to participate in this study. MP optical density was measured psychophysically, serum lutein and zeaxanthin were quantified by HPLC, and dietary intake of lutein and zeaxanthin was assessed using a val...