We recently discovered that regular alcohol consumption reduces ischemia-reperfusion injury to the same degree as ischemic preconditioning in guinea pig hearts. Ischemic preconditioning, like this cardioprotective effect of alcohol, is mediated by adenosine signaling in guinea pigs. In rats, ischemic preconditioning may be mediated predominantly by α1-adrenergic signaling. To be certain that th...

Objectives: Anti ischemic reperfusion effect of crude methanol extract of Desmodium gangeticum root had been reported earlier but how is modulating its cardiotonic effect to render cardio-protection is largely unknown. Methods: The inotropic effect of methanol extract of Desmodium gangeticum root was investigated on isolated frog heart and further analyzed the physiological and biochemical effe...

The present study was aimed to investigate the probable role of Tyrphostin AG 490, a selective inhibitor of Janus kinase-2 (JAK-2) in hyperhomocysteinemia-induced attenuation of the cardioprotective potential of ischemic postconditioning (IPOC). Rats were administered L-methionine (1.7 g/kg/day, p.o.) for 4 weeks to produce hyperhomocysteinemia. Isolated Langendorff's perfused normal and hyperh...

BACKGROUND/AIM The generation of reactive oxygen species by activated Kupffer cells (KC) may contribute to reperfusion injury of the liver during liver transplantation or resection. The aim of our present studies was to investigate (1) prevention of hepatic reperfusion injury after warm ischemia by administration of the antioxidant glutathione (GSH) and (2) whether GSH confers protection throug...

Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main source(s): Research, Development and Innovation Office Hungary (NKFIA; NVKP-16-1-2016-0017 Heart Program OTKA-FK 134751) MTA-SE System Pharmacology Research Group, Department Pharmacotherapy, Semmelweis University, H-1089 Budapest, Ischemic heart disease is the leading cause death worldwide; ...

The purpose of this study was to determine whether protein kinase C-epsilon (PKCε) and extracellular signal-regulated kinase (ERK) 1/2 were involved in morphine postconditioning (MpostC) in isolated rat hearts. The isolated rat hearts were randomly assigned into one of the following groups. Hearts in the time control (TC) group were constantly perfused with K-H buffer for 105 min. Hearts in the...

The aim of this study was to evaluate the preventive role of epigallocatechin-3 gallate (EGCG, a derivative of green tea) in ischemia/reperfusion (I/R) injury of isolated rat hearts. It has been suggested that EGCG has beneficial health effects, including prevention of cancer and heart disease, and it is also a potent antioxidant. Rat hearts were subjected to 20 min of normoxia, 20 min of zero-...

BACKGROUND AND PURPOSE Oxidative damage of mitochondrial DNA (mtDNA) in the ischemic brain is expected after ischemia/reperfusion injury. A recent study demonstrated limited patterns of mtDNA deletion in the brain after ischemia/reperfusion. We studied the ischemia/reperfusion-induced global changes of mtDNA integrity and its restoration in a rat model of transient focal ischemia in vivo. MET...

BACKGROUND Parenteral administration of recombinant human erythropoietin (rhEPO) to rats induces protection against myocardial ischemia/reperfusion injury 24 hours later. However, the mechanisms by which rhEPO mediates protection have not been determined. METHODS AND RESULTS rhEPO was perfused into isolated rat hearts over 15 minutes immediately before 30 minutes of no-flow ischemia and 45 mi...