The production and storage of explosives has resulted in the environmental accumulation of 2,4,6-trinitrotoluene (TNT). The biotransformation products of the nitroaromatic compound TNT and metabolites in mammalian cells in culture and their cytotoxicity are studied. We report after our analysis by reverse phase high performance liquid chromatography (HPLC) that the most prevalent biotransformat...

A novel antifungal protein with a molecular mass around 50 kDa was purified from seeds of Sesbania virgata (Cav.) Pers. using ammonium sulfate fractionation followed by gel filtration on a Sephadex G-75 Superfine (Sigma) column and reverse-phase high performance liquid chromatography on a C8 column. The protein, designated FP1-A, with a novel N-terminal sequence AMVHSPGG(S)FS(P), showed growth ...

A blocker of postsynaptic acetylcholine receptors was isolated from venom of the Eastern green mamba Dendroaspis angusticeps, by gel filtration, ion-exchange and reverse phase high performance liquid chromatography. The isolated component blocks neuromuscular transmission and responses to exogenously applied acetylcholine in isolated chick biventer cervices preparations. These results suggest t...

A simple, rapid and precise reverse phase high performance liquid chromatography method was developed for the analysis of Almotriptan. Chromatographic separation of Almotriptan was performed by using a kromosil C18 column (250 x 4.6mm, 5 μm) as stationary phase with a mobile phase comprising of 1% Triethyl amine : acetonitrile:methanol: 05:55:40 (v/v) at a flow rate of 1.0ml/min and UV detectio...

Acyclovir, a nucleoside analog, is thought to be specific for the human herpesviruses because it requires a virally encoded enzyme to phosphorylate it to acyclovir monophosphate. Recently, acyclovir triphosphate was shown to be a direct inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase. Here, we showed that acyclovir is an inhibitor of HIV-1 replication in CD4(+) T ...

Rhus tripartitum (Ucria) Grande leaves and fruits were investigated for their contents in phenolic compounds, essential oils, fatty acids. Chemical composition antioxidant potential of these secondary metabolites using chromatographic tools different tests. Results displayed high amounts compounds leaves, concomitant with important potentialities, probably due to richness acids flavonoids as id...

Whole human saliva possesses tremendous potential in clinical diagnostics, particularly for conditions within the oral cavity such as oral cancer. Although many have studied the soluble fraction of whole saliva, few have taken advantage of the diagnostic potential of the cells present in saliva, and none have taken advantage of proteomics capabilities for their study. We report on a novel prote...

Abbreviations: AIBN, 2,20-azo-bis-isobutyronitrile; ASE, Accelerated solvent extraction; CAR, Carboxen; DAD, Diode-array detector; DI-SPME, Direct-immersion solid-phase extraction; DSPE, Dispersive solid-phase extraction; EGDMA, Ethylene glycol dimethacrylate; ESE, Enhanced solvent extraction; ESI, Electrospray ionization; FD, Fluorescence detection; FLD, Fluorimetric detection; GC, Gas chromat...

Peptide mixtures, arising from such diverse sources as tissue extracts, urine or proteolysis, are usually fractionated and characterized by a combination of ion-exchange chromatography, gel-exclusion chromatography and/or electrophoresis. Appropriate combinations of these methods will normally yield a homogeneous product, but their costs, detection limits, analyses times and yields vary. The re...