The present study was set up to formulate a generally applicable schedule for the cellular demonstration of tumour index substances using routinely prepared histological material by a method which would also be reproducible and readable and allow simultaneous histological diagnosis. The carcinoembryonic antigen (Gold and Freedman, 1965) was used as the prime subject as high-quality reagents wer...

The first stainings were tried by A VAN LEEUWENHOEK (apparently in the years 1714-1719) and by R VIEUSSENS (about 1684) studying their objects with alcoholic saffron dyes (LEWIS FT, 1942). At last, genuine histological work began much later when T HARTIG, C WEIGERT, J GERLACH, P EHRLICH and H GIERKE started in the second half of the 19 century with their systematic studies of natural and synthe...

Background: Human neutrophil peptide-1 (HNP-1) is a commonly investigated therapeutic agent. However, its role in hypertensive left ventricular hypertrophy (HLVH) remains unclear. Methods: We measured HNP-1 levels patients with hypertension and treated HLVH rat H9c2 cell models HNP-1. Cardiomyocyte indexes (i.e., single-cell surface area, fibrosis BNP levels, β-MHC levels) were hematoxylin-eosi...

background:in the recent decade, the reverse genetics method has been broadly used for rescue of negative-stranded rna viruses from cdna or viral minigenomes. this technique has been applied to study different steps in virus replication and virus-host interactions. reverse genetics could also be implemented for design of new vaccines. the t7 rna polymerase activity as well as virus (nucleocapsi...

synovial chondromatosis affecting the glenohumeral joint is rare. treatment primarily consists of arthroscopic loose body removal and synovectomy. shoulder arthroplasty has been mentioned in the literature as a treatment option for patients with coexisting arthritis, although the results have been underreported. the case of an 84-year-old man with long standing synovial chondromatosis of the sh...

Purpose: To investigate the effects of vascular endothelial growth factor (VEGF) on yes-associated protein (YAP)/transcriptional coactivator with a PDZ-binding motif (TAZ), Hippo pathway-related transcription factor, and role YAP/TAZ induced by trabecular meshwork stimulation.Methods: Human cells were cultured treated various VEGF concentrations to verify cell cytotoxicity using CCK-8 solution....

Telomerase reverse transcriptase (TERT) vectors were transfected into bone marrow mesen- chymal stem cells (BMSCs) which then cultured and selected to establish TERT-BMSC cell lines whilst sequencing BMSCs TERT-BMSCs via transcriptome in this study explore their regulatory mechanism effect on osteogenic differentiation after TERT ectopic expres- sion sheep BMSCs. After analysing differential ge...