نتایج جستجو برای: reverse transcriptase loop mediated isothermal amplification assay

تعداد نتایج: 876614  

Journal: :Analyst 2021

Human immunodeficiency virus (HIV) rapid variant mutations challenges diagnostic assays. Our variant-tolerant isothermal nucleic acid based amplification technique enables reliable detection at the point-of-care.

2016
Gaolian Xu Hang Zhao Jonathan M Cooper Julien Reboud

We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of plugs in a capillary. Heat is provided by a water bath and the results are read by the naked eye, enabling applications in low resource settings.

2013
Junyi Xu Qiuyue Zheng Ling Yu Ran Liu Xin Zhao Gang Wang Qinghua Wang Jijuan Cao

The loop-mediated isothermal amplification (LAMP) assay indicates a potential and valuable means for genetically modified organism (GMO) detection especially for its rapidity, simplicity, and low cost. We developed and evaluated the specificity and sensitivity of the LAMP method for rapid detection of the genetically modified (GM) maize T25. A set of six specific primers was successfully design...

2015
Xuzhi Zhang Qiufen Li Xianshi Jin Cheng Jiang Yong Lu Roya Tavallaie J. Justin Gooding

Integrating loop-mediated isothermal amplification (LAMP) with capacitively coupled contactless conductivity detection (C(4)D), we have developed an electrical sensor for the simultaneous amplification and detection of specific sequence DNA. Using the O26-wzy gene as a model, the amount of initial target gene could be determined via the threshold time obtained by monitoring the progression of t...

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