Water contamination by viruses has an increasing worldwide impact on human health, and has led to requirements for accurate and quantitative molecular tools. Here, we report the first one-step reverse-transcription droplet digital PCR-based absolute quantification of a RNA virus (rotavirus) in different types of surface water samples. This quantification method proved to be more precise and mor...

Hepatitis A virus (HAV) is mainly transmitted via contaminated food or water through person-to-person contact. Here, we describe development and evaluation of a reverse transcription droplet digital PCR (RT-ddPCR) real-time (RT-qPCR) assay for detection HAV in clinical specimens. The was evaluated by assessing limit detection, precision, matrix effects, sensitivity quantitative agreement. 95 % ...

Diagnosis of Tomato spotted wilt virus (TSWV) in peanut can be accomplished by enzyme-linked immunosorbent assay (ELISA) or reverse transcription polymerase chain reaction (RT-PCR) but there has been no report of a direct comparison of the success of the two assays in evaluating infection rates of field-grown peanut. We collected peanut root samples from field-grown plants, 76 in 2006 and 48 in...

Protozoan contamination in produce is of growing importance due to their capacity cause illnesses consumers fresh leafy greens. Viability assays are essential accurately estimate health risk caused by viable parasites that contaminate food. We evaluated the efficacy reverse transcription quantitative PCR (RT-qPCR), propidium monoazide coupled with (q)PCR, and viability staining using iodide thr...

Identifying the type(s) of body fluid present in forensic casework exhibits can assist with scene reconstruction and indicate potential activity types. Confirmatory identification be achieved using mRNA-based profiling assays. This commonly involves endpoint reverse-transcription PCR (RT-PCR) capillary electrophoresis (CE) detection. In comparison, real-time quantitative RT-qPCR is more sensiti...

Reoviruses have been isolated from many aquatic animals including fish and crustaceans. Viral inclusion bodies of reovirus found in the cytoplasm hepatopancreatic cells redclaw crayfish (Cherax quadricarinatus). In past, reverse transcription polymerase chain reaction (RT-PCR) designed across other hosts was attempted to detect but no specific set primers successfully identified infected crayfi...

Abstract The COVID-19 pandemic has spread worldwide for over 2 years now. raises a significant threat to global health due its transmissibility and high pathogenicity. current standard detection method COVID-19, namely, reverse transcription–polymerase chain reaction (RT–PCR), is slow inaccurate help fight the pandemic. RT–PCR takes hours days report single test result false-negative rate. As r...

Coronavirus disease 2019 (COVID-19) is significant global treat including to pregnant woman in Indonesia.A point-of-care diagnostic tool that able early diagnose and hasa good sensitivity specificity iscritical during the pandemic. The aim of this study was describe clinical, laboratory outcomesof women with presumptive COVID-19 compare betweenCOVID-19 rapid test (lateral flow immunoassay) real...

The production of a stable cDNA copy of an unstable RNA molecule by reverse transcription is a widely used and essential technology for many important applications, such as the construction of gene libraries, production of DNA probes, and analysis of gene expression by reverse transcriptase PCR (RT-PCR). However, the synthesis of full-length cDNAs is frequently inefficient, because the RT commo...

Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA...