background : many types of cancer cells require a supply of fatty acids (fa) for growth and survival, lpl, in the presence of triglyceride-rich lipoproteins, accelerates the growth of these cells. recent evidence shows that lpl plays important roles in inflammation and obesity, implies that it is an appropriate general target for chemo preventive and chemotherapeutic agents. thus, we reported l...

background acute pancreatitis is the most common serious complication of endoscopic retrograde cholangiopancreatography (ercp) that can occasionally be fatal. multiple drugs have been examined for the prevention of this side effect, with generally uncertain results. this study is an effort to prevent this complication by the use of oral n-acetyl cysteine (nac). methods a total of 100 patients w...

an aerobic, meophilic and lipolitic bacillus.sp bacterium (lipase activity 40 u/ml) was isolated from the olive extract. this isolate was identified based on morphological and biochemical characterization and 16s rrna gene sequence. effect of the solid substrate (pomegranate seed ground, grape seed ground and coriander seed ground as a solid substrate), the substrate particle size, moisture con...

Immunoprecipitations of hepatic lipase from pulselabeled rat liver have demonstrated that hepatic lipase is synthesized in two distinct molecular weight forms, HL-I (M, = 51,000) and HL-I1 (M, = 53,000). Both forms are immunologically related to purified hepatic lipase, but not to lipoprotein lipase. HL-I and HL-11 are also kinetically related and represent different stages of intracellular pro...

Treatment of 3T3-Ll adipocytes with either an oleanolic acid gtycoside or a 2orS>-protopanaxatriol g]ycoside increased the secretion of lipoprotein lipase activity into the medium dose-dependently. At a concentration of 100pg/ml, ginsenosides Ro, Re, Rgi, and Rhi increased the secretion of lipase activity into the medium by 119, 107, 56, and 32%, respectiyely. The ratio of lipase actiyity in th...

The influence of colipase on the turbidimetric measurement of the catalytic activity of pure human pancreatic lipase (EC 3.1.1.3) and of sera from pancreatitis patients was studied. A deoxycholate-stabilized triolein emulsion served as substrate. It was found that the activity of the pure, colipase-free lipase is strongly inhibited by deoxycholate, and can be blocked completely if normal serum,...

The expression of lipase from Pseudomonas sp. strain KWI-56 (recently reclassified as Burkholderia cepacia) had been found to be dependent on an activator gene (act) downstream of its structural gene (lip). In this work, the mature lipase was synthesized in an enzymatically active form with a cell-free Escherichia coli S30 coupled transcription-translation system by expressing a recombinant lip...

The conditions, including mass ratio of PEG4000 to lipase, pH, and mass ratio of diatomites to lipase, for immobilization of Candida antarctica lipase with PEG non-covalent modification were optimized by means of the response surface methodology (RSM). The immobilized lipase specific activity in the reaction of transesterification was selected as the response value. A mathematical model was dev...

Lipoprotein lipase synthesis in adipose tissue was greater in rats fed ad libitum or refed than in fasted rats. Insulin alone and together with dexamethasone increased lipoprotein lipase synthesis in adipose tissue incubated in vitro. The changes in relative lipoprotein lipase synthesis (immunoprecipitable 35S-labelled lipoprotein lipase as a fraction of general [35S]protein after pulse-labelli...

Basic polysaccharide strongly inhibited the hydrolysis of trioleoylglycerol (TO) emulsified with phosphatidylcholine and taurocholate by either pancreatic lipase or carboxylester lipase. DEAE-Sephadex dose-dependently inhibited the hydrolysis of TO by pancreatic lipase and carboxylester lipase; however, carboxymethyl-Sephadex and Sephadex G-50 did not inhibit the hydrolysis. Polydextrose (PD), ...