The symbiotic effectiveness and nodulation competitiveness of Rhizobium leguminosarum bv. trifolii soil isolates were evaluated under nonsoil greenhouse conditions. The isolates which we used represented both major and minor nodule-occupying chromosomal types (electrophoretic types [ETs]) recovered from field-grown subclover (Trifolium subterraneum L.). Isolates representing four ETs (ETs 2, 3,...

Growth and survival of two rhizobial strains, Rhizobium leguminosarum bv. trifolii, and Mesorhizobium ciceri, were used to evaluate residues from the cork industry as potential carriers for production of rhizobial inoculants. A peat-based carrier was used as a control. 41 days after inoculation, the number of viable bacteria was high, about 109 bacteria g-1 for all carriers. Rhizobial survival ...

Free-living soybean rhizobia and Bradyrhizobium spp. (lupine) have the ability to catabolize ethanol. Of the 30 strains of rhizobia examined, only the fast- and slow-growing soybean rhizobia and the slow-growing Bradyrhizobium sp. (lupine) were capable of using ethanol as a sole source of carbon and energy for growth. Two strains from each of the other Rhizobium species examined (R. meliloti, R...

Inoculation of soil or seeds with plant growth promoting bacteria ameliorates metal toxicity to plants by changing speciation in tissues but the exact location these changes remains unknown. Knowing where occur is a critical first step establish whether are driven microbial metabolism responses. Since concentrate rhizosphere, we hypothesised steep across rhizosphere. We tested this comparing zi...

Simultaneous quantification of transcripts of the whole bacterial genome allows the analysis of the global transcriptional response under changing conditions. RNA-seq and microarrays are the most used techniques to measure these transcriptomic changes, and both complement each other in transcriptome profiling. In this review, we exhaustively compiled the symbiosis-related transcriptomic reports...

Fifty-seven strains of various Rhizobium species were analyzed by two-dimensional gel electrophoresis. Since the protein pattern on such gels is a reflection of the genetic background of the tested strains, similarities in pattern allowed us to estimate the relatedness between these strains. All group II rhizobia (slow growing) were closely related and were very distinct from group I rhizobia (...

Microbial biofertilizers are becoming an effective tool for sustainable agriculture by means of the reduction of the use of chemical fertilizers. However, the knowledge of each specific plant–microorganism interaction is essential for a correct application. In this study, we analyzed the in vitro plant-growth-promotion mechanisms of a Rhizobium leguminosarum strain named PEPV16 isolated from Ph...

Identification of Rhizobium and Bradyrhizobium strains and especially of indigenous isolates continues to be one of the major difficulties associated with competition studies. Because there is no universally accepted method, the method of choice depends on preference, experience, and equipment. Here, an agar plate technique was used to distinguish strains and field isolates of Rhizobium legumin...