Extrachromosomal circular DNAs (eccDNAs) are common genetic elements in Saccharomyces cerevisiae and are reported in other eukaryotes as well. EccDNAs contribute to genetic variation among somatic cells in multicellular organisms and to evolution of unicellular eukaryotes. Sensitive methods for detecting eccDNA are needed to clarify how these elements affect genome stability and how environment...

Fluorescent labeling of nucleic acids with high density is very important for a wide area of DNAand RNAbased diagnostics [1–5], but it still remains a challenge. Indeed, chemical synthesis of oligonucleotides with multiple Xuorophores is an elaborate and expensive procedure [6–8]. Branched probes tagged with numerous Xuorophores can alleviate this problem [2,9], but special complex assemblies a...

DNA methylation is a crucial epigenetic modification and is closely related to tumorigenesis. Herein, a surface ligation-based high throughput method combined with bisulfite treatment is developed for analysis of methylated genomic DNA. In this method, a DNA microarray is employed as a reaction platform, and resonance light scattering (RLS) of nanoparticles is used as the detection principle. T...

We have demonstrated the isothermal in vitro amplification and multimerization of several different linear DNA targets using only two primers and the strongly strand-displacing exonuclease-negative Bst DNA polymerase. This reaction has been termed linear target isothermal multimerization and amplification (LIMA). LIMA has been compared with cascade rolling-circle amplification and has been foun...

introduction: plant diseases caused by geminiviruses are one of the main constraints of legume production in the world. they are responsible for a constraint on production of various crops. geminiviruses are characterized by their twinned icosahedral particles and their single-stranded dna genome. the family geminiviridae is grouped into four genera: begomovirus, mastrevirus, curtovirus and top...

Fluorescent labeling of nucleic acids with high density is very important for a wide area of DNAand RNAbased diagnostics [1–5], but it still remains a challenge. Indeed, chemical synthesis of oligonucleotides with multiple Xuorophores is an elaborate and expensive procedure [6–8]. Branched probes tagged with numerous Xuorophores can alleviate this problem [2,9], but special complex assemblies a...

Numts are an integral component of many eukaryote genomes offering a snapshot of the evolutionary process that led from the incorporation of an α-proteobacterium into a larger eukaryotic cell some 1.8 billion years ago. Although numt sequence can be harnessed as molecular marker, these sequences often remain unidentified and are mistaken for genuine mtDNA leading to erroneous interpretation of ...

Sporothrix infections are emerging as an important human and animal threat among otherwise healthy patients, especially in Brazil and China. Correct identification of sporotrichosis agents is beneficial for epidemiological surveillance, enabling implementation of adequate public-health policies and guiding antifungal therapy. In areas of limited resources where sporotrichosis is endemic, high-t...

Chronic Hepatitis B Virus (HBV) infections can progresses to liver cirrhosis and hepatocellular carcinoma (HCC). The HBV covalently-closed circular DNA cccDNA is a key to HBV persistence, and its degradation can be induced by the cellular deaminase APOBEC3. This study aimed to measure the distribution of intrahepatic cccDNA levels and evaluate the association between levels of cccDNA and APOBEC...

Yam (Dioscorea spp.) plants are potentially hosts to a diverse range of badnavirus species (genus Badnavirus, family Caulimoviridae), but their detection is complicated by the existence of integrated badnavirus sequences in some yam genomes. To date, only two badnavirus genomes have been characterised, namely, Dioscorea bacilliform AL virus (DBALV) and Dioscorea bacilliform SN virus (DBSNV). A ...