Bacterial membranes serve as selective environmental barriers and contain determinants required for bacterial colonization and survival. Cell envelopes of Gram-negative bacteria consist of an outer and an inner membrane separated by a periplasmic space. Most Gram-negative bacteria display a smooth outer surface (e.g., Enterobacteriaceae), whereas members of the Pasteurellaceae and Moraxellaceae...

CRISPR/Cas12a-based detection is a novel approach for the efficient, sequence-specific identification of viruses. Here we adopt use CRISPR/Cas12a to identify tomato brown rugose fruit virus (ToBRFV), new and emerging tobamovirus which causing substantial damage global industry. Specific CRISPR RNAs (crRNAs) were designed detect either ToBRFV or closely related mosaic (ToMV). This technology ena...

Florida is a leading producer of fresh-market tomatoes (Solanum lycopersicum) in the US, $262 million industry for state. In October 2019, eight tomato plants showing chlorotic, cupped leaves and stunted foliage (Fig. 1) were collected from c. 1.6 ha community garden Alachua County, Florida. The symptoms observed characteristic Tomato yellow leaf curl virus (TYLCV). Samples tested by PCR using ...

Abstract Tomato brown rugose fruit virus (ToBRFV) is a new member of the genus Tobamovirus , and has potential to affect production marketability tomatoes peppers. In this study, we sequenced analyzed complete genome ToBRFV isolates from tomato plants showing mosaic mottling symptoms in Yunnan Province China. We constructed full-length infectious cDNA clone ToBRFV, which could induce systemic i...