Congenital Lumbar spinal stenosis (LSS) defined first by Dr. Sarpyener in 1940’s17, and degenerative LSS was defined by Verbiest in 1950’s.19 Degenerative LSS can be classified as simple or complex LSS, depending on the presence or absence of attendant pathologies. LSS can also be classified on axial plane as central, lateral, or foraminal. LSS may result inneurogenic claucation or radiculopath...

Breakup of loosely bound nuclei as indirect method in nuclear astrophysics: Abstract. We discuss the use of one-nucleon breakup reactions of loosely bound nuclei at intermediate energies as an indirect method in nuclear astrophysics. These are peripheral processes, therefore we can extract asymptotic normalization coefficients (ANC) from which reaction rates of astrophysical interest can be inf...

The amino acid sequences of two ribosomal proteins, S14 and S16, from the archaebacterium Halobacterium marismortui have been determined. Sequence data were obtained by the manual and solid-phase sequencing of peptides derived from enzymatic digestions with trypsin, chymotrypsin, pepsin, and Staphylococcus aureus protease as well as by chemical cleavage with cyanogen bromide. Proteins S14 and S...

The mosquito Aedes aegypti (Ae. aegypti) is the most notorious vector of illness-causing viruses such as Dengue, Chikugunya, and Zika. Although numerous genetic expression studies utilizing quantitative real-time PCR (qPCR) have been conducted with regards to Ae. aegypti, a panel of genes to be used suitably as references for the purpose of expression-level normalization within this epidemiolog...

BACKGROUND AND OBJECTIVES Gene manipulation and cell vaccines represent innovative strategies to enhance the immunogenicity of cancer cells. We adopted a defective lentivirus derived from the human immunodeficiency virus (HIV)-1 backbone and carrying the enhanced green fluorescent protein (EGFP) gene to transduce primary human acute myelogenous leukemia (AML) and B-precursor acute lymphoblastic...

1. Lueck S, Angeli E. Challenge of neonatal myocardial protection (letter). Ann Thorac Surg 2013;96:2286–7. 2. Bojan M, Peperstraete H, Lilot M, Tourneur L, Vouh e P, Pouard P. Cold histindine-tryptophan-ketoglutarate solution and repeated oxygenated warm blood cardioplegia in neonates with arterial switch operation. Ann Thorac Surg 2013;95: 1390–6. 3. Buckberg GD. Myocardial temperature manage...

Four lipid-rich microalgal species from the Red Sea belonging to three different genera (Nannochloris, Picochlorum and Desmochloris), previously isolated as novel biodiesel feedstocks, were bioprospected for high-value, bioactive molecules. Methanol extracts were thus prepared from freeze-dried biomass and screened for different biological activities. Nannochloris sp. SBL1 and Desmochloris sp. ...

Human embryonic stem (ES) cells are undifferentiated, pluripotent cells that can be maintained indefinitely in culture. Here we demonstrate that human ES cells differentiate to hematopoietic precursor cells when cocultured with the murine bone marrow cell line S17 or the yolk sac endothelial cell line C166. This hematopoietic differentiation requires fetal bovine serum, but no other exogenous c...

We constructed a family of conditionally replicating plasmids, the pTX1 family, which are based on the IncPalpha oriV origin of replication that is dependent on the trfA-encoded protein. We constructed several Escherichia coli derivatives expressing trfA from different chromosomal loci, which can be transduced by phage P1 to any E. coli strain. The pTX1 plasmids also carry the oriTRP4 origin of...

To correlate a prokaryotic endosymbiont in the pea aphid, Acyrthosiphon kondoi, with the endosymbionts in related aphid species as well as with free-living bacteria and subcellular organelles, and to study the mode of its gene expression within aphid cells, we have cloned and characterized the genes encoding ribosomal proteins S3, L16, L29, S17, L14, L24, L5, S14, S8, L6, L18, S5, L30, L15 and ...