Bone marrow-derived mesenchymal stromal stem cells (BMSCs) are a promising cell source for treating articular cartilage defects. The objective of this study was to assess the impact of cell seeding density within a collagen I scaffold on in vitro BMSC chondrogenesis following isolation and expansion in two-dimensional (2D) and three-dimensional (3D) environments. It was hypothesized that both e...

Our approach to cartilage tissue-engineering scaffolds combines image-based design and solid free-form (SFF) fabrication to create load-bearing constructs with user-defined parameters. In this study, 3-dimensional scaffolds with cubic and ellipsoidal pore architecture were fabricated using poly(propylene fumarate) (PPF). To increase seeding efficiency and cellular retention, hydrogels were used...

OBJECTIVES To investigate whether a combination of demineralized bone matrix (DBM) and bone marrow mesenchymal stem cells (BMSCs) infected with adenovirus-mediated- bone morphogenetic protein (Ad-BMP-2) and transforming growth factor-β3 (Ad-TGF-β3) promotes the repair of the full-thickness cartilage lesions in pig model. METHODS BMSCs isolated from pig were cultured and infected with Ad-BMP-2...

Abstract In the current study, a novel nanomaterial called nanomagnetite/copper oxide/potassium carrageenan nanocomposite (MKCO) was fabricated to include Fenton (nanomagnetite, NM) and Fenton-like reagent (copper oxide nanoparticles, NCO) in matrix of potassium biopolymer. The prepared solid materials were characterized by different physicochemical techniques, such as TGA, N 2 adsorption/desor...

In this study, it was aimed to immunohistochemically evaluate the possible effect of probiotic supplementation on rats fed a high cholesterol diet mast cell heterogeneity and density in urinary bladder expression chymase, tryptase tumor necrosis factor-alpha (TNF-α) cytokine bladder. Three groups were formed study. For 8 weeks, Group 1 (control group) regular rat diet. Food for 2 (feeding +2% c...

Dwarf shrubs are a key functional group of the high-elevation vegetation belt. Despite their ecological relevance and high sensitivity to environmental changes, hydraulic adaptations species-specific variations in drought tolerance within this growth form mostly unknown. Here, we assess xylem vulnerability cavitation important character species Alpine dwarf shrub heaths Central Europe. Due perc...

Water contaminated with low concentrations of pollutants is more difficult to clean up than that high pollutant content levels. Membrane separation provides a solution for removing from water. However, membranes are prone fouling, losing performances over time. Here we synthesized neutral (IM-NH2) and positively charged (IL-NH2) imidazole derivatives chemically functionalize membranes. With dis...

Objective: In the present study, we investigated effects of cinnamon on distribution and number mast cells (MCs) in liver tissue diabetic rats. Material Methods: The rats used as control group received no application. All diabetes diabetes+cinnamon groups were made by a single dose intraperitoneal injection streptozotocin. our after establishment diabetes, extract was administered daily oral ga...

In a classification of the aerobic spore-forming bacilli proposed by Smith and Clark (1937) one of the important differential points is the presence of reserve fat in the cells of organisms of the "B. cereus-B. megatherium group" when grown on glucose agar. In contrast, bacilli of the "B. subtilis-B. mesentericus group," and some other sub-groups, are said not to store fat. The authors do not d...