The role of the Salmonella Pathogenicity Islands (SPIs) in pathogenesis of Salmonella enterica Typhimurium infection in the chicken is poorly studied, while many studies have been completed in murine models. The Type VI Secretion System (T6SS) is a recently described protein secretion system in Gram-negative bacteria. The genus Salmonella contains five phylogenetically distinct T6SS encoded in ...

Genetic analysis in bacteria often involves mapping novel mutations. Traditionally, mapping has been performed using time-consuming methods such as conjugation and transductional analysis. However, an alternative strategy is to use physical mapping using restriction enzymes, which cleave bacterial chromosomal DNA at relatively few sites, generating DNA fragments that can be resolved by pulsed-f...

Most Salmonella enterica serovar Typhimurium strains are capable of infecting multiple hosts. In this report, an abdominal cavity abscess associated with the Salmonella enterica serovar Typhimurium phage type DT2 is described in a dog. A dead male dog was admitted to the Department of Pathology at the Faculty of Veterinary Medicine at Erciyes University for necropsy. Anorexia, weight loss and l...

The gastroenteritis-causing pathogen Salmonella typhimurium induces profound transcriptional changes in intestinal epithelia resulting in the recruitment of neutrophils whose presence is the histopathologic hallmark of salmonellosis. Here we used cDNA microarray expression profiling to define the molecular determinants that mediate such changes in model intestinal epithelia. Enteropathogenic Sa...

Invasive Salmonella infections for which improved or new vaccines are being developed include enteric fever caused by Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B and sepsis and meningitis in young children in sub-Saharan Africa caused by nontyphoidal Salmonella (NTS) serovars, particularly S. enterica serovars Typhimurium and Enteritidis. Assays are needed to measure functi...

Salmonella enterica ssp. enterica is a leading cause of bacterial food-borne disease outbreaks worldwide and is also an economic burden particularly in Reunion Island because its population consumes large amounts of chicken and cooks 100% chicken sausages (35 kg per capita per year). The aim of this study was to investigate the epidemiology of Salmonella Typhimurium and Salmonella 1,4,[5],12:i:...

Objective: Teucrium polium L. (family Lamiaceae) is a wild-growing flowering plant, found abundantly in South-Western Asia, Europe and North Africa. Traditionally, T. polium has been used for different pathological conditions such as gastrointestinal disorders, inflammations, diabetes and rheumatism. The purpose of this study was to evaluate the antimicrobial effect of ethanol extract of Teucri...

Salmonella Typhimurium is one of the most common foodborne pathogens isolated from poultry meat. The goal this study was to investigate S. survival in broiler carcasses exposed ozone, lactic acid, sodium hypochlorite and levulinic acid. inoculated into carcasses, which were divided eight treatment groups, including a positive negative control group. After standardized bacterial culture methods,...

E. coli and Salmonella typhimurium are widely used bacterial hosts for genetic manipulation of DNA from prokaryotes and eukaryotes. Introduction of foreign DNA by electroporation or transduction into E. coli and Salmonella is limited by host restriction of incoming DNA by the recipient cells. Here, we describe a simple method that temporarily inactivates host restriction, allowing high-frequenc...

Salmonella is one of the major pathogenic bacteria that cause food poisoning. To elucidate the host infection mechanism of Salmonella enterica serovar Typhimurium-targeting phages, the bacteriophage SPN3UB was isolated from a chicken fecal sample. This phage belongs morphologically to the Siphoviridae family and infects the host via the O antigen of lipopolysaccharide (LPS). To further understa...