The perception and response to cellular death is an important aspect of multicellular eukaryotic life. For example, damage-associated molecular patterns activate an inflammatory cascade that leads to removal of cellular debris and promotion of healing. We demonstrate that lysis of Pseudomonas aeruginosa cells triggers a program in the remaining population that confers fitness in interspecies co...

Hepatic lipase (HL) is a secretory protein synthesized in hepatocytes and bound to liver endothelium. Previous studies have suggested that HL N-linked glycans are required for catalytic activity. To directly test this hypothesis, Xenopus laevis oocytes were used to express native rat HL or HL lacking one or both N-linked glycosylation sites. The expressed and secreted native HL had an apparent ...

A 25-year-old man was found to have a large right adrenal mass detected by abdominal echography and computed tomography, and presented with a mild gynecomastia. Endocrine study showed increased serum concentrations and urinary excretion of estrogens and dehydroepiandorosterone sulfate (DHEA-S). The patient had no Cushingoid features but autonomous cortisol secretion, compatible with the diagnos...

Non-invasive ventilation (NIV) for acute exacerbations of chronic obstructive pulmonary disease (COPD) has been shown to reduce both morbidity and mortality in this population when compared with endotracheal intubation. NIV may also be used in a host of other diseases including cardiogenic pulmonary edema, post-extubation hypoxemia, and chronic ventilatory failure associated with muscular weakn...

A surgical procedure was developed for autoplastic grafting of the ovary into the splenic circulation. The ovarian transplant is joined to the spleen by an attached piece of oviduct and uterus. Direct arterial connections to the graft are maintained throughout the procedure; there is no direct contact of splenic and ovarian tissue. Transplantation of the left utero-tubo-ovarian segment and remo...

Non-invasive ventilation (NIV) for acute exacerbations of chronic obstructive pulmonary disease (COPD) has been shown to reduce both morbidity and mortality in this population when compared with endotracheal intubation. NIV may also be used in a host of other diseases including cardiogenic pulmonary edema, post-extubation hypoxemia, and chronic ventilatory failure associated with muscular weakn...

Secretion of human soluble tumor necrosis factor receptor type I (sTNFRI) by the mouse fibrosarcoma cell line, L929, previously has been demonstrated to confer resistance to in vitro lysis by TNF and to LAK- and CTL-mediated cytolysis. These findings suggest that, in vivo, sTNFRI contributes to tumor survival by inhibiting these immunologic mechanisms. To evaluate this hypothesis, we compared t...

Intracellular Mycobacterium leprae infection modifies host macrophage programming, creating a protective niche for bacterial survival. The milieu regulating cellular apoptosis in the tissue plays an important role in defining susceptible and/or resistant phenotypes. A higher density of apoptotic cells has been demonstrated in paucibacillary leprosy lesions than in multibacillary ones. However, ...

In summarizing the findings of this paper it may be said that degenerative changes have been noted in practically every parenchymatous organ. Among these the most striking has been that of serous imbibition by the most active cells of these organs. In regard to the changes in the glands of internal secretion, the findings corroborate the statements of Cushing in regard to hypophysectomy, that r...

The AvrPphB effector of Pseudomonas syringae is a papain-like protease that is injected into the host plant cell and cleaves specific kinases to disrupt immune signaling. Here, we used the unique substrate specificity of AvrPphB to generate a specific activity-based probe. This probe displays various AvrPphB isoforms in bacterial extracts, upon secretion and inside the host plant. We show that ...