We investigated the contribution of opening of the ATP-sensitive K+ channel to extracellular accumulation of K+ during ischemia with the use of glibenclamide, a specific blocker of this K+ channel. To characterize the electrophysiological effects of glibenclamide during metabolic inhibition (by either application of dinitrophenol or hypoxia) we performed patch-clamp studies in isolated membrane...

Two unlinked recessive mutations (ks-1 and ks-2 ) have been induced in Paramecium tetraurelia stock 51. Wild-type survives and grows when up to 30 mm KCl is added to the medium, but the mutants cease to grow and die when added KCl reaches 20-25 m m. These K(+)-sensitives have been crossed to stocks containing the K(+)-resistant genes, fA (very resistant) and kA (moderately resistant). All four ...

BACKGROUND Resveratrol is a polyphenolic compound extracted from plants and is also a constituent of red wine. Resveratrol produces relaxation of vascular smooth muscle and may prevent cardiovascular diseases. Although resveratrol has been reported to cause relaxation of the guinea pig gallbladder, limited data are available about the effect of resveratrol on the gallbladder smooth muscle in hu...

This study aims to determine the levels of macro minerals such as potassium, calcium, sodium, and magnesium in fresh noni fruit. The sample fruit was destructed, then quantitative analysis were performed using atomic absorption spectrophotometry (AAS) at a wavelength 766.5 nm for potassium; 422.7 calcium; 589.0 sodium; 285.2 magnesium. advantages this method are selective sensitive. average % r...

BACKGROUND AND PURPOSE During cerebral ischemia, both hypoxia and hypercapnia appear to produce marked dilatation of the cerebral arteries. Hypercapnia and hypoxia may be accompanied by extracellular and intracellular acidosis, which is another potent dilator of cerebral arteries. However, the precise mechanism by which acidosis produces dilatation of the cerebral arteries is not fully understo...

The intercalated (IC) cells of the cortical collecting duct (CCD) are important to acid-base homeostasis by secreting acid and reabsorbing bicarbonate. Acid secretion is mediated predominantly by apical membrane Schering (SCH-28080)-sensitive H(+)-K(+)- ATPase (HKA) and bafilomycin-sensitive H(+)-ATPase. The SCH-28080-sensitive HKA is believed to be the gastric HKA (HKAg). Here we examined apic...

Neuronal firing is regulated by the complex interaction of multiple depolarizing and hyperpolarizing currents; intrinsic firing, which defines the neuronal ability to generate action potentials in the absence of synaptic excitation, is particularly sensitive to modulation by currents that are active below the action potential threshold. Cerebellar unipolar brush cells (UBCs) are excitatory gran...

BACKGROUND AND PURPOSE This study examined the hypotheses that (1) atherosclerosis impairs relaxation of the carotid artery in response to activation of adenosine triphosphate (ATP)-sensitive potassium channels and (2) regression of atherosclerosis restores the response toward normal. METHODS Isometric tension was measured in rings of carotid artery taken from normal, atherosclerotic, and reg...

Potassium-selective ion channels are important for accurate transmission of signals from auditory and vestibular sensory end organs to their targets in the central nervous system. During different gravity conditions, astronauts experience altered input signals from the peripheral vestibular system resulting in sensorimotor dysfunction. Adaptation to altered sensory input occurs, but it is not e...