The sensitivity and specificity of counterimmunoelectrophoresis using three antigenic preparations obtained from Salmonella typhi were compared with those of the Widal test in the serological diagnosis of typhoid fever. A veronal buffer extract yielded precipitation lines against 50 (96%) out of 52 sera collected from patients with typhoid but against none out of 62 sera obtained from control s...

The objective of this study was to compare the performance of different diagnostic tests for the detection of pigs subclinically infected with Actinobacillus pleuropneumoniae. To address this question, we infected pigs with APP serotypes 1, 3, 5, 7, 10, 12, and 15 and collected weekly tonsil swabs for isolation and PCR and serum samples for serological testing. Our results showed that the Multi...

The author describes the case of a 3-year-old boy presenting with cough and fever that had been persisting for seven days not respondent to amoxicillin. Neutrophilic leukocytosis diarrhoea were also present. diagnosis adenovirus infection was finally made, confirmed by serological test.

Monoclonal antibodies to human immunoglobulin M (IgM) were used in a four-phase enzyme immunofluorescence "capture" assay for determination of IgM antibodies to measles and rubella viruses. Little or no background reactivity was seen in the test system, and interfering effects of rheumatoid factor were avoided by preabsorption of test sera with aggregated human IgG. Virus-specific IgM antibody ...

We performed a comparatÍve study bebween xenodiagnosis and serological tests for Chagasfdisease. 150 Patients from several endemic areas were studied. Four of them appeared to have a peculiar status with positive xenodiagnosis and negative serclogy carr'ied out with four classical techniques (Immunofluorescence test, ELrsA: Enzyme Linked rmmunosorbent Assay, complement fixation test and Imrnuno...

A survey of caseous lymphadenitis was conducted at a goat and sheep slaughterhouse in Northeastern Brazil One hundred and fifty-eight goats and 43 sheep were examined for the presence of abscesses, with bacterial culturing of purulent material to define the etiological agent. Blood was collected simultaneously for determination of serological titer via the synergistic hemolysis-inhibition test ...

One hundred sera from 49 patients with glandular toxoplasmosis were examined by a latex agglutination test, the dye test, an indirect haemagglutination test, and a double antibody sandwich enzyme linked immunosorbent assay (ELISA) for antitoxoplasma IgM. The results support previous findings that the dye test, indirect haemagglutination test, and latex agglutination test measure different antib...

A comparison of the direct agglutination test and the indirect hemagglutination test for the detection of blood serum antibodies to Escherichia coli organisms indicated that these serological tests were comparable. In some instances the indirect hemagglutination test provided higher endpoint readings. Preparation of the antigens for the indirect hemagglutination test was more time consuming tha...

A presumptive hemagglutination test for the serological diagnosis of leptospirosis in humans is described. The antigen was prepared from a soluble alcohol extract of an andamana strain sorbed to human O-negative erythrocytes and preserved by pyruvic aldehyde fixation. In this study, the overall sensitivity of the hemagglutination test was 92% in contrast to 69% for the presumptive slide aggluti...