The aim of this study was to investigate the effect of reproductive hormones in seminal plasma on the quality of the spermatozoa in men seeking infertility treatment. Of the 383 volunteers recruited, 123 had normal sperm parameters and 260 had either a single or a combination of sperm abnormalities as per WHO criteria. Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) Prolactin (PRL)...

Sexual contact with HIV-infected semen is a major driving force behind the global HIV pandemic. Little is known regarding the immune correlates of virus shedding in this compartment, although HIV-1-specific CD8+ T cells are present in semen. We collected blood and semen from 27 chronically HIV-infected, therapy-naive men without common sexually transmitted infections or urethral inflammation an...

Laboratory validation is essential in developing an effective method for separating X and Y sperm to preselect sex. Utilizing sexed sperm from a particular experiment to test fertility and achieve the subsequent phenotypic sex without knowing the likely outcome at conception is too costly for most applications. Further, research advances need to be built on an ongoing assessment with respect to...

Results: Serum samples became PCR-positive before semen samples. During the first 6 days after inoculation, serum was PRRSV-positive in 36 of 40 boars, and semen was PRRSV-positive in four of 40 boars. Median time to detection was 36 and 48 hours for nested PCR and Taqman PCR, respectively. Results were inconsistent when a positive semen sample was pooled with negative semen. Elevated rectal te...

The effect of gelatin addition to the semen extender on the viability and acrosome integrity of rabbit spermatozoa was studied. Pooled semen samples were processed in a boar semen extender with or without gelatin addition. Semen samples were stored at 5 degrees C for 72 h. Viability and acrosome integrity was evaluated by light microscope. Results showed that gelatin addition had a significant ...

The screening and confirmatory tests available to a forensic laboratory allow evidence to be examined for the presence of bodily fluids. With the majority of evidence being submitted involving sexual assaults, it is important to have confirmatory tests for the identification of semen that are straightforward, quick, and reliable. The purpose of this study was to compare two commonly used semen ...

A total of 120 ejaculates from six adult Black Bengal bucks were studied to determine the semen attributes and evaluate their fertility. The various semen attributes of fresh semen were volume, sperm concentration, mass motility, live spermatozoa and normal spermatozoa percentage. All the semen attributes differed significantly (P<0.05) except normal spermatozoa percentage between the bucks. A ...

BACKGROUND Acute lung injury (ALI) is an inflammatory disorder. Semen Cassiae has potent anti-inflammatory activities. The aim of our study was to investigate whether Semen Cassiae plays a protective effect on lipopolysaccharide (LPS)-induced ALI and, if so, to elucidate its potential mechanism. METHODS Male Sprague-Dawley rat lungs were injured by intratracheal instillation of LPS. Rats were...

OBJECTIVE To determine the prevalence of herpes viruses in the semen of an asymptomatic male cohort with and without infertility problems and its association with altered semen parameters. DESIGN A prospective randomized study. SETTING Medical school and IVF clinic. PATIENT(S) One hundred seventy-two male patients undergoing routine semen analysis: 80 with normal semen parameters (control...

The sperm quality index (SQI) is a tool used to predict overall rooster semen quality, fertility, and hatchability. However, semen must be diluted before SQI analysis, and research has shown that the SQI is most predictive of fertility at lower semen dilutions. Therefore, the present study was undertaken to determine why the SQI is not as predictive of fertility at higher semen dilutions and wh...