Based on the sequences of the bovine amelogenin genes, we have designed a protocol for sexing DNA samples of wild ruminants. Basically the protocol consists on the co-amplification of two specific fragments, one from Ychromosome and one for the X chromosome, making the use of a PCR control unnecessary. It has been demonstrated to be useful for sex identification in a total of 164 samples belong...

Methods: During the first 10 weeks after an outbreak of acute porcine reproductive and respiratory syndrome (PRRS) in a herd but prior to implementing McREBEL management, crossfostering was performed only within 24 hours of birth; however, because the manager was sizing and sexing all litters at birth, > 85% of piglets were being raised by a foster dam. Under the McREBEL protocol, crossfosterin...

Producers of domestic livestock strive to improve genetic influences in their herds. This requires identification, and propagation of animals that demonstrate desirable characteristics. The more animals available from which to select, the greater the opportunity to discover high-performance animals. Predetermination of the sex of offspring would provide a greater number of males or females from...

Background Sexing sperm is an important biotechnical that enables improve animal production, with achievement of maximum animal breeding. However, all sex semen methodologies can promote irreparable damages to sperm cell, which compromise their fertility [1]. Among the main causes of injures to the sperm are the reactive oxygen species, which have their production intensified during semen manip...