optimization of the condition for pcr-directed sequencing of microsatellites poly adenine (a) length polymorphisms is more difficult and sensitive compared with other common sequences. replication slippage may occur for polymerase enzyme during microsatellite amplification and direct sequencing of these pcr products will be challenging for heterozygote samples. so, the aim of this study is to i...

Quite some bones of cows and pigs are discharged from home and restaurant industry as food wastes after cooking by boiling. This study conducted adsorption experiment of heavy metals using waste bones as an absorbent for recycling the waste bone and evaluated its feasibility. Sintered cattle bone (SCB) was manufactured in powder form after sintering at 550 ̊C 600 ̊C and pulverizing, and crystal s...

BACKGROUND Osteoarthritis (OA) is a clinically important and common disease of older cats. The pathological changes and molecular mechanisms which underpin the disease have yet to be described. In this study we evaluated selected histological and transcriptomic measures in the articular cartilage and subchondral bone (SCB) of the humeral condyle of cats with or without OA. RESULTS The histomo...

Saccharomyces mating-type switching occurs through a double-strand break-initiated gene conversion event at MAT, using one of two donors located distantly on the same chromosome, HMLalpha and HMRa. MATa cells preferentially choose HMLalpha, a decision that depends on the recombination enhancer (RE) that controls recombination along the left arm of chromosome III. We previously showed that an fh...

BACKGROUND Hydrogen is regarded as an attractive future energy carrier for its high energy content and zero CO2 emission. Currently, the majority of hydrogen is generated from fossil fuels. However, from an environmental perspective, sustainable hydrogen production from low-cost lignocellulosic biomass should be considered. Thermophilic hydrogen production is attractive, since it can potentiall...

Sugarcane bagasse (SCB), one of the major lignocellulosic agro-industrial waste products, was used as a substrate for biosynthesis of polyhydroxyalkanoates (PHA) by halophilic archaea. Among the various wild-type halophilic archaeal strains screened, Halogeometricum borinquense strain E3 showed better growth and PHA accumulation as compared to Haloferaxvolcanii strain BBK2, Haloarcula japonica ...

Dolichyl phosphate mannose synthetase (GDP-mannose: dolichyl-phosphate O-beta-D-mannosyltransferase; EC 2.4.1.83) is an enzyme that is involved in glycoconjugate biosynthesis and possesses a putatively conserved dolichol binding site. In order to probe the interaction between the enzyme and the dolichol chain, lipid phosphates varying in length and extent of branching have been tested as substr...

Abstract Breakwaters are the structures constructed in coastal areas to maintain calm inside port or prevent beach erosion. Semi-circular Breakwater (SCB) is an innovative type of made hollow caisson on a base slab with without perforations. In this study, wave overtopping discharge parameter SCB estimated using Artificial Neural Network and Random Forest. The data collected used current resear...

The present study reports an anomalous branching pattern of the thoracic sympathetic chain. At the level of T3 ganglion, an anomalous branch i.e accessory sympathetic chain (ASC) descended anteromedial to the main sympathetic chain (MSC). The MSC and the ASC communicated with each other at the level of T9, T10 and T11 ganglion, indicating the absence of classical pattern of greater, lesser and ...

Topological entanglements between polymer chains are achieved in associating protein hydrogels through the synthesis of high molecular weight proteins via chain extension using a variety of thiol coupling chemistries, including disulfide formation, thiol-maleimide, thiol-bromomaleimide and thiol-ene. Coupling of cysteines via disulfide formation results in the most pronounced entanglement effec...