Gain or loss of repeat motifs leads to an allelic mismatch in the disputed child which is a deviation from Mendelian inheritance, thereby leading paternal putative father exclusion mother case maternal mismatch. This at one more loci major cause forensic inferences. The biological samples were genotyped using Powerplex®- Fusion 5C system kit and Investigator® Argus X-12 QS as per recommendation...

Formation of deletions by recombination between short direct repeats is thought to involve either a break-join or a copy-choice process. The key step of the latter is slippage of the replication machinery between the repeats. We report that the main replicase of Escherichia coli, DNA polymerase III holoenzyme, slips between two direct repeats of 27 bp that flank an inverted repeat of approximat...

Schedule slippage plagues the space industry, and is antinomic with the recent emphasis on space responsiveness. The Government Accountability Office has repeatedly noted the difficulties encountered by the Department of Defense in keeping its acquisition of space systems on schedule, and identified the low Technology Readiness Level (TRL) of the system/payload under development as a principal ...

Human fingertip and its derivatives in robotics possess an inhomogeneous structure with skin, tissue, bone, and nail; which causes troublesome for construction of a precise model that can represent almost mechanical characteristics, especially during sliding motion. Commonly, models in a virtual haptic environment are frictionless, thus attempts to display tactile sensations are still remained ...

STUDY DESIGN Retrospective study. PURPOSE To compare initial fixation using the cortical bone trajectory (CBT) technique versus conventional pedicle screws (PS) in radiographs of postsurgical lumbar degenerative spondylolisthesis. OVERVIEW OF LITERATURE Few reports have documented the holding strength of CBT technique for spondylolisthesis cases. METHODS From October 2009 to June 2014, 21...

Tracer chloride and potassium net efflux from valinomycin-treated human erythrocytes were measured into media of different chloride concentrations, Clo, at 25 degrees C and pH 7.8. Net efflux was maximal [45-50 mmol (kg cell solids)-1 min-1] at Clo = 0. It decreased hyperbolically with increasing Clo to 14-16 mmol (kg cell solids)-1 min-1. Half-maximal inhibition occurred at Clo = 3 mM. In the ...

Accumulation of Ca(2+) by the Ca(2+)-ATPase of skeletal-muscle sarcoplasmic reticulum has been measured in reconstituted, sealed vesicles as a function of lipid composition. Measurements were performed in the presence of carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) to eliminate any effects of H(+) transport; in the presence of FCCP, addition of valinomycin had no effect on the leve...

In this, the first of three papers, we present the sequence of the ribosomal RNA (rRNA) genes of Drosophila melanogaster. The gene regions of D. melanogaster rDNA encode four individual rRNAs: 18S (1,995 nt), 5.8S (123 nt), 2S (30 nt), and 28S (3,945 nt). The ribosomal DNA (rDNA) repeat of D. melanogaster is AT rich (65.9% overall), with the spacers being particularly AT rich. Analysis of DNA s...

The large amount of nanoscale pores in shale results in the inability to apply Darcy's law. Moreover, the gas adsorption of shale increases the complexity of pore size characterization and thus decreases the accuracy of flow regime estimation. In this study, an apparent permeability model, which describes the adsorptive gas flow behavior in shale by considering the effects of gas adsorption, st...

Tandem repeats (TRs) are often present in proteins with crucial functions, responsible for resistance, pathogenicity and associated with infectious or neurodegenerative diseases. This motivates numerous studies of TRs and their evolution, requiring accurate multiple sequence alignment. TRs may be lost or inserted at any position of a TR region by replication slippage or recombination, but curre...