N-Acetylneuraminic acid aldolase from Glostridium perfringens was irreversiblv inactivated by 1 mM-bromopyruvate with a half-life of 4.2min at pH 7.2 and 370C. The rate of inactivation was diminished in the presence of pyruvate but not with N-acetyl-D-mannosamine, indicating that the inhibitor acted at, or close to, the pyruvate-binding site. The apparent K, for bromopyruvate, calculated from t...