Plant-associated microbes play important roles in global ecology and agriculture. The most common method to profile these microbial communities is amplicon sequencing of the bacterial 16S ribosomal RNA (rRNA) gene. Both DNA extraction PCR amplification steps this process are subject bias, especially because latter requires some way exclude from plant organelles, which would otherwise dominate s...

Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common soil bacterium Pseudomonas stutzeri, isolated from aquifer cores and from human spinal fluid were exposed to ultraviolet light. Amplification length polymorphism analysis (AFLP) was used to genotype this bacterial species and evaluate th...

Abstract By conducting numerical simulations of Treasure Island site in 1989 Loma Prieta earthquake, the Hilbert-Huang transform was used to study soil liquefaction. The UBC3D-PLM constitutive model simulate dynamic behavior sand, non-liquefied simulated by Hardening Small Strain model, and seismic information recorded PEER database for deconvolution analysis one-dimensional equivalent linear m...

This article presents a staggered grid time-domain finite-difference (FD) program for the simulation of sH-wave propagation in a viscoelastic heterogeneous medium. The incorporation of realistic damping in FD program is based on a rheological model widely known as generalized maxwell body (gmB-EK). The accuracy of implementation of realistic damping is validated by comparing the numerically com...

Six isolates of Bacillus thuringiensis isolated from soil samples confirmed to be toxic to mosquito larvae were differentiated using a PCR-Based technique. Three of these isolates initially identified using a serological technique were further differentiated with the PCR amplification of the delta-endotoxin target sequences. Using the total DNA of isolates as template, at least four isolates yi...

Possible nonlinearity in the response of sedimentary layers to incident P waves is studied using the strong-motion data f rom a vertical array in Taiwan at the site where a significant nonlinear response to S waves had been found. Soil transfer functions are analyzed at different excitation levels with a maximum vertical acceleration at surface reaching 0 . 1 g, corresponding to the compression...

The first GCM climate change projections to include dynamic vegetation and an interactive carbon cycle produced a very significant amplification of global warming over the 21st century. Under the IS92a ‘‘business as usual’’ emissions scenario CO2 concentrations reached about 980 ppmv by 2100, which is about 280 ppmv higher than when these feedbacks were ignored. The major contribution to the in...

Pseudomonas putida is a Gram-negative, rod-shaped, saprotrophic soil bacterium. Based on 16S rRNA analysis, P. putida was taxonomically confirmed to be a Pseudomonas species. Faster detection of the causative Microbes and institution of proper therapy would help greatly in preventing septic complications. Recent advances in the field of Molecular Biology, including the amplification of genetic ...