In vitro cultures of human bone marrow have been used to study normal haemopoiesis and to gain information to aid in the treatment of leukaemia patients. A workshop on the development of semi-solid bone marrow cultures was held for two days in Washington on 15 and 16 August 1976. The participants of this workshop discussed some of the technical problems associated with semi-solid cultures of hu...

AIM We investigated whether homologous recombinant deletion of the endothelial cell-specific protein Von Willebrand factor (vWF) affected hematopoiesis in long-term bone marrow cultures, and irradiation induction of pulmonary fibrosis/organizing alveolitis. MATERIALS AND METHODS We established long-term bone marrow cultures from vWF(-/-) (C57BL/6 background) and vWF(+/+) littermate mice. Non-...

This article includes a series of methods for culturing the moss Physcomitrella patens at all stages of its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage of gametophytes, cultures are maintained on solid medium at 10°C in a very short day. Cryopreservation may also be used. Finally, sporophytes are generated by se...

Modified solid-state fermentation (MSSF) of tannin-rich substrates for production of tannase and gallic acid was carried out using fungal co-cultures. Tannin acyl hydrolase or tannase is an enzyme produced extracellularly in solid state fermentation by various fungi. The solid-state fermentation offers a number of advantages over submerged and liquid conventional fermentation for the enzyme pro...

Abstract In the development of all‐solid‐state lithium batteries (ASSLB), progress is made with solid‐state electrolytes; however, challenges regarding compatibility and stability still exist solid electrodes. These issues result in a low battery capacity short cycle life, which limit commercial application ASSLBs. This review summarizes recent research on electrodes ASSLBs including solid–soli...