This work studies the feasibility to employ a combination of volcanic ash (natural waste) with different raw materials in production geopolymers: fly and mining tailing (considered hazardous solid waste), natural pozzolan, metakaolin. study compares properties geopolymers based on ash, metakaolin, relation 1:1 addition NaOH 15M Na2SiO3 as alkali activators. FTIR XRD assays mechanical tests were...

Alkali-activated binders have been considered a low carbon alternative to cement and are produced by reacting aluminosilicate precursor with an alkali-activator. However, alkali-activators observed be major contributor the environmental burdens of alkali-activated materials regarding various impact categories. Therefore, this study aims perform assessment using life cycle methodology on mortars...

1. Polyinosinic acid (poly(1)) is capable of releasing the DNA template restrictions of rat liver nuclei or soluble chromatin when assayed with Escherichia coli DNA polymerase. In contrast, polycytidylic acid (poly(C)) is completely ineffective. 2. The ability of poly(1) to release the DNA template restrictions is dependent on the size of the poly(1). Poly(1) with sedimentation values from 4.1 ...

1. Polyinosinic acid (poly(1)) is capable of releasing the DNA template restrictions of rat liver nuclei or soluble chromatin when assayed with Escherichia coli DNA polymerase. In contrast, polycytidylic acid (poly(C)) is completely ineffective. 2. The ability of poly(1) to release the DNA template restrictions is dependent on the size of the poly(1). Poly(1) with sedimentation values from 4.1 ...

BACKGROUND Transcription by RNA polymerase II in eukaryotic cells requires the ordered assembly of general transcription factors on the promoter to form a preinitiation complex. Transcriptional activator proteins (activators) stimulate transcription by increasing the rate and/or extent of preinitiation complex assembly. We have shown previously that acidic activators increase the stable associa...

DNA methylation and chromatin modification operate along a common pathway to repress transcription; accordingly, several experiments demonstrate that the effects of DNA methylation can spread in cis and do not require promoter modification. In order to investigate the molecular details of the inhibitory effect of methylation, we microinjected into Xenopus oocytes a series of constructs containi...

Cell-based splicing of minigenes is used extensively in the analysis of alternative splicing events. In particular, such assays are critical for identifying or confirming the in vivo relevance of cis- and trans-acting factors in the regulation of particular splicing patterns. Here we provide detailed information on the methods specific to the cell-based analysis of minigene splicing. In additio...

Cyclin dependent protein kinases (CDKs) play vital role in gene expression and cell cycle regulation. CDKs require cyclin binding activity, phosphorylation through CDK activating kinase (CAK), Cdc25, Wee 1 kinase. Non-cyclin CDK activators include CDK5 activators, Viral Cyclins and RINGO/Speedy. Among all CDK activators, CAK carries prime importance. The time frame of activating phosphorylation...

Antiproliferative protein (APP) isolated from conditioned media of two androgen-independent prostatic carcinoma cell lines, PC3 and Du-145 was shown to inhibit selectively cell proliferation of androgen-dependent prostate cancer cell line LNCaP in a dose dependent manner. This protein was further purified with HPLC using hydrophobic interaction column (phenyl 5PW) and was used to study the modu...

Regulation of transcription is vitally important for maintaining normal cellular homeostasis and is also the basis for cellular differentiation, morphogenesis and the adaptability of any organism. Transcription activators, which orchestrate time and locus-specific assembly of complex transcription machinery, act as key players in these processes. One way in which these activators are controlled...