To fertilize eggs, mammalian sperm must first undergo a form of cellular exocytosis called the acrosome reaction. In 1983, my laboratory reported that nanomolar concentrations of ZP3, one of three zona pellucida glycoproteins from mouse eggs, induced mouse sperm to undergo the acrosome reaction in vitro. Seven years later, this article from Florman’s laboratory (O’Toole et al., 2000) revealed t...

Ocean acidification caused by the uptake of anthropogenic CO2 in the oceans negatively affects the early life stages of corals by reducing their calcification rate. Acidification also inhibits the sperm motility of corals, potentially affecting fertilization success. We investigated the effects of different pCO2 (partial pressure of CO2) conditions on the sperm motility of Acropora digitifera. ...

A combination of flow cytometric sperm sorting of X and Y chromosome-bearing sperm (X and Y sperm) and computer-assisted sperm analysis (CASA) for measuring sperm motility allows assessment of motion parameters in the two populations. Bull sperm were separated into X and Y populations by flow cytometry following staining with the DNA-binding dye Hoechst 33,342. The motion parameters differed de...

This study investigated the effects of skim-milk supplementation on the quality and penetrating ability of boar semen preserved at 15 °C. When boar semen samples were preserved in Modified Modena extender supplemented with various concentrations (0, 7.5, 15, 30 and 50 mg/mL) of skim milk powder at 15 °C for 4 weeks, higher sperm motility and viability were observed in the case of 7.5 mg/mL skim...

OBJECTIVE To investigate a possible protective role of lycopene on adriamycin (ADR)-induced spermiotoxicity using quantitative, biochemical and histopathological approaches. DESIGN Experimental study. SETTING Firat University Medical School, Experimental Research Centre, Elazig, Turkey. ANIMALS Twenty four Sprague Dawley rats (8-weeks old) INTERVENTION(S) Adriamycin (10 mg kg(-1)) was i...

Male Wistar rats were exposed to ethylene oxide (EO) at concentrations of 50, 100, or 250 ppm for six hours a day, on five days a week for 13 weeks. Dose effect relations of inhaled EO on spermatogenesis were evaluated from testicular and epididymal weights, histopathological changes and lactate dehydrogenase X (LDH X) activity in the testis, and sperm counts and sperm head abnormalities in the...

objectives: the aim of the present study was to evaluate protective effect of royal jelly on sperm parameters, testosterone level, and malondialdehyde (mda) production in mice. materials and methods: thirty-two adult male nmri mice weighing 30±2 g were used. all the animals were divided into 4 groups. control group: received saline 0.1 ml/mouse/day orally for 30 days. royal jelly group (rj): re...

This report focuses on the development of a method for chemically induced enhancement of cell elution and recovery from cotton swabs. The method exploits the exclusive use of detergents for intact cell removal, and can be utilized in conjunction with, or to circumvent, conventional differential extraction (DE). Samples treated with Sarkosyl (54.4 +/- 1.8%) and sodium dodecyl sulfate (SDS) (78.5...

Mesoscale structures (microns dimensions, nL-pL volumes) have been designed and fabricated in silicon for use in various analytical tasks. We studied sperm motility and performed sperm selection in channels (80 microns wide x 20 microns deep), branching structures (40 microns wide x 20 microns deep, eight bifurcations), and channels containing barriers (7 microns feature size). Sperm-cervical m...

Sperm of salmonid fishes are quiescent in the presence of millimolar concentrations of extracellular K+, but motility initiation occurs when sperm are suspended in K+-free medium. In this study, glycerol (CH2OHCHOHCH2OH) treatment of intact sperm in the presence of K+ induced the initiation of motility even though a large amount of K+ was present. Another organic alcohol, erythritol (CH2OH(CHOH...