We tested whether capacitation, a phenomenon that renders spermatozoa capable of undergoing acrosomal exocytosis, may be completed rapidly after a short exposure to bicarbonate/CO2. We found that, in the presence of Ca2+, a 10-min exposure of boar spermatozoa to bicarbonate led to a partial activation of phospholipase A2, primed spermatozoa for a major subsequent activation of this enzyme upon ...

The hydrodynamic basis for the accumulation of spermatozoa at surfaces has been investigated. The general conclusion is that when spermatozoa arrive at a surface, they will remain there if the vector of the time-averaged thrust is directed towards that surface. This can arise in two basic ways. First, consider spermatozoa that maintain a three-dimensional waveform and roll (spin) as they progre...

Although dog prostatic fluid decreases the longevity of ejaculated dog spermatozoa, it also increases their rate of motility and their fertility after vaginal insemination, as well as the fertility of epididymal spermatozoa after uterine insemination. These findings indicate a need to further characterize the effects of prostatic fluid on dog spermatozoa. This study was done to determine the ef...

background: the lipid peroxides and their degradation products are highly toxic to spermatozoa and may play a major role in sperm dysfunction. the purpose of this study was to determine the level of lipid peroxidation as indicated by malondialdehyde (mda) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males. in addition, correlation of lipid peroxidation with mot...

Background: Epididymal spermatozoa have been used for production of offspring from genetically important animals after they are dead. To attain optimal fertility with epididymal spermatozoa, they should be preserved in a suitable medium in 37°C. Preservation media provides requirement for spermatozoa and affects its motility, viability, and fertility. Sucrose is one of the important components ...

Background: The lipid peroxides and their degradation products are highly toxic to spermatozoa and may play a major role in sperm dysfunction. The purpose of this study was to determine the level of lipid peroxidation as indicated by malondialdehyde (MDA) in the spermatozoa and seminal plasma of asthenozoospermic and normozoospermic males. In addition, correlation of lipid peroxidation with mot...

BACKGROUND Relaxin levels in seminal plasma have been associated with positive effects on sperm motility and quality, and thus having potential roles in male fertility. However, the origin of seminal relaxin, within the male reproductive tract, and the moment of its release in the vicinity of spermatozoa remain unclear. Here, we assessed the longitudinal distribution of relaxin and its receptor...

Mammalian spermatozoa experience osmotic stress when the glycerol is added to the cells before freezing and removal from the cells after thawing. To minimize osmotic damage, alternative cryoprotectants, having lower molecular weights and greater membrane permeability than glycerol, were evaluated to determine their effectiveness for cryopreserving bull spermatozoa. The primary goal of this stud...

The objective of this study was to evaluate the effect of reducing sperm numbers per insemination dose on fertility of cryopreserved buffalo bull semen. For this purpose, semen was collected at weekly intervals from a Nili-Ravi buffalo bull (Bubalus bubalis) using an artificial vagina in two batches. The ejaculates were split-sampled and diluted at 37°C with tris-citric acid extender having 15x...

Successful fertilization requires viable and functional spermatozoa to recognize and fuse with the oocyte. In most mammalian species, mature spermatozoa are not capable of fertilizing the oocytes immediately after ejaculation. However, unlike somatic cells, spermatozoa, after leaving the testis, are transcriptionally and translationally silent; therefore, upon completion of spermiogenesis, sper...