Pericytes play an important role in modulating angiogenesis, but the origin of these cells is poorly understood. To evaluate whether the mature vessel wall contains pericyte progenitor cells, nonendothelial mesenchymal cells isolated from the rat aorta were cultured in a serum-free medium optimized for stem cells. This method led to the isolation of anchorage-independent cells that proliferated...

Cells from the prostate tumor cell line LNCaP have been grown as spheroids. The growth kinetics of the spheroids have been characterized by fitting a Gompertz equation to spheroid growth curves. The proliferation state of cells within spheroids of different diameters was assessed by bromodeoxyuridine staining. Scanning and electron transmission microscopy were performed to determine the ultrast...

A microfluidic system provides an excellent platform for cellular studies. Most importantly, a three-dimensional (3D) cell culture model reconstructs more accurately the in vivo microenvironment of tissue. Accordingly, microfluidic 3D cell culture devices could be ideal candidates for in vitro cell culture platforms. In this paper, two types of 3D cellular aggregates, i.e., toroid and spheroid,...

PURPOSE The humanized monoclonal antibody, trastuzumab (Herceptin), directed against HER2/neu, has been effective in the treatment of breast cancer malignancies. However, clinical activity has depended on HER2/neu expression. Radiolabeled trastuzumab has been considered previously as a potential agent for radioimmunotherapy. The objective of this study was to investigate the efficacy of trastuz...

Spheroids are generally self-assembled cells with the ability to generate their extracellular matrix, including complex cell-matrix and cell-cell interactions that resemble functional characteristics of corresponding tissue in vivo. The study aimed develop a three-dimensional (3D) spheroid system for cervical cancer cell lines, HeLa (HPV18), CaSki (HPV16), SiHa C33A (non-HPV), HT3 (non-HPV) as ...

The differing Mbh-L relations presented in McLure & Dunlop, Marconi & Hunt and Erwin et al. have been investigated. A number of issues have been identified and addressed in each of these studies, including but not limited to: the removal of a dependency on the Hubble constant; a correction for dust attenuation in the bulges of disc galaxies; the identification of lenticular galaxies previously ...

The use of biomarkers to infer drug response in patients is being actively pursued, yet significant challenges with this approach, including the complicated interconnection of pathways, have limited its application. Direct empirical testing of tumor sensitivity would arguably provide a more reliable predictive value, although it has garnered little attention largely due to the technical difficu...

Three-dimensional cell culture has many advantages over monolayer cultures, and spheroids have been hailed as the best current representation of small avascular tumours in vitro. However their adoption in regular screening programs has been hindered by uneven culture growth, poor reproducibility and lack of high-throughput analysis methods for 3D. The objective of this study was to develop a me...

Like many other solid tumors, cervical cancer contains a heterogeneous population of cancer cells. Several investigators have identified putative stem cells from solid tumors and cancer cell lines via the capacity to self renew and drive tumor formation. The aim of this study was to identify and characterize a cancer stem-like cell population from primary carcinoma of the cervix uteri. Cervical...

PTEN (phosphatase and tensin homolog), a tumour suppressor negatively regulating the PI3K signalling pathway, is the second most frequently mutated gene in human cancers. Decreased PTEN expression is correlated with colorectal cancer metastases and poor patient survival. Three dimensional (3D) multicellular spheroid models have been postulated to bridge the gap between 2D cell models and animal...